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Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT
Lv, Zhao1,2,3; Qiu, Limei1; Wang, Mengqiang1; Jia, Zhihao1,3; Wang, Weilin1,3; Xin, Lusheng1,3; Liu, Zhaoqun1,3; Wang, Lingling2,4; Song, Linsheng2,4
2018
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume78Pages:42-51
SubtypeArticle
AbstractC1q domain containing proteins (C1qDCs) are a family of proteins containing a globular head C1q domain (ghC1q) in C-terminus, which serve as pattern recognition receptors (PRRs) and mediate a series of immune responses. In the present study, three C1qDC proteins from pacific oyster Crassostrea gigas(CgC1qDC-2, CgC1qDC-3, CgC1qDC-4) were characterized and comparatively investigated to understand their roles in the immune response. All the three recombinant CgC1qDC proteins (rCgC1qDCs) could bind lipopolysaccharide (LPS) significantly but they could not bind Iipoteichoic acid (LTA), beta-1,3-glucan (GLU), mannan (MAN), and polyinosinic-polycytidylic acid (Poly I:C). Correspondingly, they all exhibited higher binding activities towards Gram-negative bacteria Vibrio anguillarum and V. splendidus. Moreover, they could enhance the phagocytosis of oyster hemocytes, and the enhancements towards Gram-negative bacteria were significantly higher than that towards Gram-positive bacteria (p < 0.01). The LPS binding affinity of rCgC1qDC-3 (K-D = 8.74 x 10(-7) M) was higher than that of rCgC1qDC-2 (K-D = 7.76 x 10(-5) M) and rCgC1qDC-4 (K-D = 1.09 x 10(-5) M). Meanwhile, rCgC1qDC-3 exhibited significantly higher enhancement on phagocytosis of oyster hemocytes towards Gram-negative bacteria than that of rCgC1qDC-2 and rCgC1qDC-4 (p < 0.05). After the secondary challenge with V. splendidus, the up-regulations of CgC1qDC-2 and CgC1qDC-4 mRNA in hemocytes occurred at 6 h, while that of CgC1qDC-3 was observed at 3 h and lasted for 24 h. And CgC1qDC-3 responded with high mRNA level for tested 24 h upon the secondary challenge with V. anguillarum as well. These results collectively suggested that three CgC1qDCs could serve as PRRs to specifically recognize certain Gram-negative bacteria and opsonins to enhance phagocytosis. CgC1qDC-3, with higher binding affinity to LPS, stronger opsonization and more rapid and persistent mRNA expression response upon the secondary challenge with homologous Vibrios, might exert efficient functions in the immune responses against invading pathogens. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordC1qdc Proteins Gram-negative Bacteria Recognition Specificity Lps Binding Affinity
DOI10.1016/j.dci.2017.09.014
Indexed BySCI
Language英语
WOS IDWOS:000413881000005
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143079
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Lv, Zhao,Qiu, Limei,Wang, Mengqiang,et al. Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,78:42-51.
APA Lv, Zhao.,Qiu, Limei.,Wang, Mengqiang.,Jia, Zhihao.,Wang, Weilin.,...&Song, Linsheng.(2018).Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,78,42-51.
MLA Lv, Zhao,et al."Comparative study of three C1q domain containing proteins from pacific oyster &ITCrassostrea gigas&IT".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 78(2018):42-51.
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