| Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities | |
Hou, Ningning1,2,3 ; Zhang, Meng4,5; Xu, Yingjie5; Sun, Zhongmin3,6; Wang, Jing1,3; Zhang, Lijuan5; Zhang, Quanbin1,3
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| 2017-12-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 卷号 | 105页码:1511-1518 |
| 文章类型 | Article |
| 摘要 | Crude polysaccharides from Costaria costata were extracted by hot water and further fractionated by anion exchange chromatography into three polysaccharide fractions. Three low molecular weight fragments were then prepared by degradation of the polysaccharides with hydrogen peroxide and ascorbic acid. The structural features of the polysaccharides and their low molecular weight fragments were elucidated for the first time based on the HGPC, FT-IR, NMR, MS, monosaccharide composition, and other chemical analyses. Their anticoagulant and FGF-1, -2, -7, -8, -9, -10/FGFR1c signaling activation activities in BaF3 cells were also examined. Our studies showed that the polysaccharides were sulfated at different positions of galactose and fucose residues. The APTT-, PT- and TT-based anticoagulant assay results indicated that a high molecular weight and a higher degree of sulfation were essential for their anticoagulant activities. In contrast, not only the polysaccharides but also the depolymerized fragments showed significant FGF/FGFR signal activating activities in a FGF-, molecular weight-, and sulfation-dependent manner. The results presented in current study demonstrated the potential use of the polysaccharides and their fragments as anticoagulants and FGF signal regulators. (C) 2017 Elsevier B.V. All rights reserved. |
| 关键词 | Costaria Costata Polysaccharide Structure Anticoagulant Fibroblast Growth Factor (Fgf) Fibroblast Growth Factor Receptor (Fgfr) |
| DOI | 10.1016/j.ijbiomac.2017.06.042 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000415779800019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143072 |
| 专题 | 海洋生物分类与系统演化实验室 实验海洋生物学重点实验室 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China 5.Qingdao Univ, Affiliated Hosp, Inst Cerebrovasc Dis, Qingdao 266003, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Hou, Ningning,Zhang, Meng,Xu, Yingjie,et al. Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,105:1511-1518. |
| APA | Hou, Ningning.,Zhang, Meng.,Xu, Yingjie.,Sun, Zhongmin.,Wang, Jing.,...&Zhang, Quanbin.(2017).Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,105,1511-1518. |
| MLA | Hou, Ningning,et al."Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 105(2017):1511-1518. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Polysaccharides and （3973KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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