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Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities
Hou, Ningning1,2,3; Zhang, Meng4,5; Xu, Yingjie5; Sun, Zhongmin3,6; Wang, Jing1,3; Zhang, Lijuan5; Zhang, Quanbin1,3
2017-12-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume105Pages:1511-1518
SubtypeArticle
AbstractCrude polysaccharides from Costaria costata were extracted by hot water and further fractionated by anion exchange chromatography into three polysaccharide fractions. Three low molecular weight fragments were then prepared by degradation of the polysaccharides with hydrogen peroxide and ascorbic acid. The structural features of the polysaccharides and their low molecular weight fragments were elucidated for the first time based on the HGPC, FT-IR, NMR, MS, monosaccharide composition, and other chemical analyses. Their anticoagulant and FGF-1, -2, -7, -8, -9, -10/FGFR1c signaling activation activities in BaF3 cells were also examined. Our studies showed that the polysaccharides were sulfated at different positions of galactose and fucose residues. The APTT-, PT- and TT-based anticoagulant assay results indicated that a high molecular weight and a higher degree of sulfation were essential for their anticoagulant activities. In contrast, not only the polysaccharides but also the depolymerized fragments showed significant FGF/FGFR signal activating activities in a FGF-, molecular weight-, and sulfation-dependent manner. The results presented in current study demonstrated the potential use of the polysaccharides and their fragments as anticoagulants and FGF signal regulators. (C) 2017 Elsevier B.V. All rights reserved.
KeywordCostaria Costata Polysaccharide Structure Anticoagulant Fibroblast Growth Factor (Fgf) Fibroblast Growth Factor Receptor (Fgfr)
DOI10.1016/j.ijbiomac.2017.06.042
Indexed BySCI
Language英语
WOS IDWOS:000415779800019
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/143072
Collection海洋生物分类与系统演化实验室
实验海洋生物学重点实验室
海洋生物技术研发中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China
5.Qingdao Univ, Affiliated Hosp, Inst Cerebrovasc Dis, Qingdao 266003, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Hou, Ningning,Zhang, Meng,Xu, Yingjie,et al. Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,105:1511-1518.
APA Hou, Ningning.,Zhang, Meng.,Xu, Yingjie.,Sun, Zhongmin.,Wang, Jing.,...&Zhang, Quanbin.(2017).Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,105,1511-1518.
MLA Hou, Ningning,et al."Polysaccharides and their depolymerized fragments from Costaria costata: Molecular weight and sulfation-dependent anticoagulant and FGF/FGFR signal activating activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 105(2017):1511-1518.
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