A teleost CD46 is involved in the regulation of complement activation and pathogen infection

doi:10.1038/s41598-017-15124-y

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	A teleost CD46 is involved in the regulation of complement activation and pathogen infection
	Li, Mo-fei1,2 ; Sui, Zhi-hai 1,3; Sun, Li 1,2
	2017-11-03
发表期刊	SCIENTIFIC REPORTS
卷号	7
文章类型	Article
摘要	In mammals, CD46 is involved in the inactivation of complement by factor I (FI). In teleost, study on the function of CD46 is very limited. In this study, we examined the immunological property of a CD46 molecule (CsCD46) from tongue sole, a teleost species with important economic value. We found that recombinant CsCD46 (rCsCD46) interacted with FI and inhibited complement activation in an FI-dependent manner. rCsCD46 also interacted with bacterial pathogens via a different mechanism to that responsible for the FI interaction, involving different rCsCD46 sites. Cellular study showed that CsCD46 was expressed on peripheral blood leukocytes (PBL) and protected the cells against the killing effect of complement. When the CsCD46 on PBL was blocked by antibody before incubation of the cells with bacterial pathogens, cellular infection was significantly reduced. Consistently, when tongue sole were infected with bacterial pathogens in the presence of rCsCD46, tissue dissemination and survival of the pathogens were significantly inhibited. These results provide the first evidence to indicate that CD46 in teleosts negatively regulates complement activation via FI and protects host cells from complement-induced damage, and that CD46 is required for optimal bacterial infection probably by serving as a receptor for the bacteria.
DOI	10.1038/s41598-017-15124-y
收录类别	SCI
语种	英语
WOS记录号	WOS:000414414700001
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/143051
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Mo-fei,Sui, Zhi-hai,Sun, Li. A teleost CD46 is involved in the regulation of complement activation and pathogen infection[J]. SCIENTIFIC REPORTS,2017,7.
APA	Li, Mo-fei,Sui, Zhi-hai,&Sun, Li.(2017).A teleost CD46 is involved in the regulation of complement activation and pathogen infection.SCIENTIFIC REPORTS,7.
MLA	Li, Mo-fei,et al."A teleost CD46 is involved in the regulation of complement activation and pathogen infection".SCIENTIFIC REPORTS 7(2017).

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