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| Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles | |
Zeng, Yan1 ; Zhang, Dun1; Qi, Peng1; Zheng, Laibao1,2
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| 2017-12-01 | |
| 发表期刊 | MICROCHIMICA ACTA
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| 卷号 | 184期号:12页码:4809-4815 |
| 文章类型 | Article |
| 摘要 | The authors describe a strategy for colorimetric detection of DNA. It is making use of a target catalyzed DNA nanostructure assembly and gold nanoparticles. The assay comprises the following steps: (a) A programmed DNA nanostructure is assembled from three auxiliary hairpin structure DNAs and the single stranded DNA (ssDNA; the target/analyte); (b) in the presence of target DNA, these three hairpin DNAs are opened, thereby activating a catalytic self-assembly process via a target assisted toe-hold strand displacement reaction; (c) The formed DNA nanostructures are mixed with gold nanoparticles. As the DNA nanostructure is less stabilized without ssDNA sticky ends, it cannot prevent the gold nanoparticles (AuNPs) to undergo salt-induced aggregation which is accompanied by a color change from purple to blue; (d) The color change of the colloid solution can be read out with bare eyes or instrumentally. The detection limit by using UV-vis spectrometry is 0.6 pM of target DNA. This is comparable to previously AuNP-based methods. Thus, this assay provides free modification detection based on DNA nanostructure without sophisticated procedures. Conceivably, the method can be applied to numerous other DNA targets. |
| 关键词 | Dnadetection Goldnanoparticle Colorimetry Self-assembly Dna Nanostructure |
| DOI | 10.1007/s00604-017-2463-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000414547400029 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/143036 |
| 专题 | 海洋环境腐蚀与生物污损重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Zeng, Yan,Zhang, Dun,Qi, Peng,et al. Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles[J]. MICROCHIMICA ACTA,2017,184(12):4809-4815. |
| APA | Zeng, Yan,Zhang, Dun,Qi, Peng,&Zheng, Laibao.(2017).Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles.MICROCHIMICA ACTA,184(12),4809-4815. |
| MLA | Zeng, Yan,et al."Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles".MICROCHIMICA ACTA 184.12(2017):4809-4815. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Colorimetric detecti（1538KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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