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Effects of Lugol's iodine solution and formalin on cell volume of three bloom-forming dinoflagellates
Yang Yang1,2; Sun Xiaoxia1,3; Zhao Yongfang1
2017-07-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume35Issue:4Pages:858-866
SubtypeArticle
AbstractFixatives are traditionally used in marine ecosystem research. The bias introduced by fixatives on the dimensions of plankton cells may lead to an overestimation or underestimation of the carbon biomass. To determine the impact of traditional fixatives on dinoflagellates during short- and long-term fixation, we analyzed the degree of change in three bloom-forming dinoflagellates (Prorocentrum micans, Scrippsiella trochoidea and Noctiluca scintillans) brought about by Lugol's iodine solution (hereafter Lugol's) and formalin. The fixation effects were species-specific. P. micans cell volume showed no significant change following long-term preservation, and S. trochoidea swelled by approximately 8.06% in Lugol's and by 20.97% in formalin as a percentage of the live cell volume, respectively. N. scintillans shrank significantly in both fixatives. The volume change due to formalin in N. scintillans was not concentration-dependent, whereas the volume shrinkage of N. scintillans cells fixed with Lugol's at a concentration of 2% was nearly six-fold that in cells fixed with Lugol's at a concentration of 0.6%-0.8%. To better estimate the volume of N. scintillans fixed in formalin at a concentration of 5%, we suggest that the conversion relationship was as follows: volume of live cell=volume of intact fixed cell/0.61. Apart from size change, damage induced by fixatives on N. scintillans was obvious. Lugol's is not a suitable fixative for N. scintillans due to high frequency of broken cells. Accurate carbon biomass estimate of N. scintillans should be performed on live samples. These findings help to improve the estimate of phytoplankton cell volume and carbon biomass in marine ecosystem.
KeywordCell Volume Formalin Lugol's Noctiluca Scintillans
DOI10.1007/s00343-017-5378-0
Indexed BySCI
Language英语
WOS IDWOS:000403701200016
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/137145
Collection胶州湾海洋生态系统国家野外研究站
海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Marine Ecosyst Res Stn, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang Yang,Sun Xiaoxia,Zhao Yongfang. Effects of Lugol's iodine solution and formalin on cell volume of three bloom-forming dinoflagellates[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(4):858-866.
APA Yang Yang,Sun Xiaoxia,&Zhao Yongfang.(2017).Effects of Lugol's iodine solution and formalin on cell volume of three bloom-forming dinoflagellates.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(4),858-866.
MLA Yang Yang,et al."Effects of Lugol's iodine solution and formalin on cell volume of three bloom-forming dinoflagellates".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.4(2017):858-866.
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