Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages | |
Hui Min1; Cui Zhaoxia1,2,3; Liu Yuan1; Song Chengwen1,4
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| 2017-07-01 | |
| Source Publication | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| Volume | 35Issue:4Pages:770-781 |
| Subtype | Article |
| Abstract | In crab, embryogenesis is a complicated developmental program marked by a series of critical events. RNA-Sequencing technology offers developmental biologists a way to identify many more developmental genes than ever before. Here, we present a comprehensive analysis of the transcriptomes of Eriocheir sinensis oosperms (Os) and embryos at the 2-4 cell stage (Cs), which are separated by a cleavage event. A total of 18 923 unigenes were identified, and 403 genes matched with gene ontology (GO) terms related to developmental processes. In total, 432 differentially expressed genes (DEGs) were detected between the two stages. Nine DEGs were specifically expressed at only one stage. These DEGs may be relevant to stage-specific molecular events during development. A number of DEGs related to 'hedgehog signaling pathway', 'Wnt signaling pathway' 'germplasm', 'nervous system', 'sensory perception' and 'segment polarity' were identified as being up-regulated at the Cs stage. The results suggest that these embryonic developmental events begin before the early cleavage event in crabs, and that many of the genes expressed in the two transcriptomes might be maternal genes. Our study provides ample information for further research on the molecular mechanisms underlying crab development. |
| Keyword | Mitten Crabs Embryo Development Fertilized Egg Cleavage Transcriptomics |
| DOI | 10.1007/s00343-017-5364-6 |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000403701200007 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/137129 |
| Collection | 实验海洋生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Hui Min,Cui Zhaoxia,Liu Yuan,et al. Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(4):770-781. |
| APA | Hui Min,Cui Zhaoxia,Liu Yuan,&Song Chengwen.(2017).Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(4),770-781. |
| MLA | Hui Min,et al."Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.4(2017):770-781. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Transcriptome profil（829KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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