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Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)
Song, Hao1,2; Dang, Xin3; He, Yuan-qiu3; Zhang, Tao1,4; Wang, Hai-yan1,4
2017-05-31
发表期刊PEERJ
卷号5
文章类型Article
摘要Background. The veined rapa whelk Rapana venosa is an important commercial shellfish in China and quantitative real-time PCR (qRT-PCR) has become the standard method to study gene expression in R. venosa. For accurate and reliable gene expression results, qRT-PCR assays require housekeeping genes as internal controls, which display highly uniform expression in different tissues or stages of development. However, to date no studies have validated housekeeping genes in R. venosa for use as internal controls for qRT-PCR.
关键词Internal Control Real-time Pcr Tissue Development Rapana Venosa
DOI10.7717/peerj.3398
收录类别SCI
语种英语
WOS记录号WOS:000402560400008
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/137105
专题海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
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Song, Hao,Dang, Xin,He, Yuan-qiu,et al. Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)[J]. PEERJ,2017,5.
APA Song, Hao,Dang, Xin,He, Yuan-qiu,Zhang, Tao,&Wang, Hai-yan.(2017).Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa).PEERJ,5.
MLA Song, Hao,et al."Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)".PEERJ 5(2017).
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