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Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)
Song, Hao1,2; Dang, Xin3; He, Yuan-qiu3; Zhang, Tao1,4; Wang, Hai-yan1,4
2017-05-31
Source PublicationPEERJ
Volume5
SubtypeArticle
AbstractBackground. The veined rapa whelk Rapana venosa is an important commercial shellfish in China and quantitative real-time PCR (qRT-PCR) has become the standard method to study gene expression in R. venosa. For accurate and reliable gene expression results, qRT-PCR assays require housekeeping genes as internal controls, which display highly uniform expression in different tissues or stages of development. However, to date no studies have validated housekeeping genes in R. venosa for use as internal controls for qRT-PCR.
KeywordInternal Control Real-time Pcr Tissue Development Rapana Venosa
DOI10.7717/peerj.3398
Indexed BySCI
Language英语
WOS IDWOS:000402560400008
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/137105
Collection海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Song, Hao,Dang, Xin,He, Yuan-qiu,et al. Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)[J]. PEERJ,2017,5.
APA Song, Hao,Dang, Xin,He, Yuan-qiu,Zhang, Tao,&Wang, Hai-yan.(2017).Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa).PEERJ,5.
MLA Song, Hao,et al."Selection of housekeeping genes as internal controls for quantitative RT-PCR analysis of the veined rapa whelk (Rapana venosa)".PEERJ 5(2017).
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