IOCAS-IR  > 实验海洋生物学重点实验室
Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus
Xiu, Pengyuan1,2,3; Liu, Rui1,2; Zhang, Dechao4; Sun, Chaomin1,2
2017-06-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume83Issue:12
SubtypeArticle
AbstractBacterial motility is a crucial factor during the invasion and colonization processes of pathogens, which makes it an attractive therapeutic drug target. Here, we isolated a marine bacterium (Vibrio alginolyticus strain 178) from a seamount in the tropical West Pacific that exhibits vigorous motility on agar plates and severe pathogenicity to zebrafish. We found that V. alginolyticus 178 motility was significantly suppressed by another marine bacterium, Bacillus sp. strain 176, isolated from the same niche. We isolated, purified, and characterized two different cyclic lipopeptides (CLPs) from Bacillus sp. 176 using high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance spectroscopy. The two related CLPs have a pumilacidin-like structure and were both effective inhibitors of V. alginolyticus 178 motility. The CLPs differ by only one methylene group in their fatty acid chains. In addition to motility suppression, the CLPs also induced cell aggregation in the medium and reduced adherence of V. alginolyticus 178 to glass substrates. Notably, upon CLP treatment, the expression levels of two V. alginolyticus flagellar assembly genes (flgA and flgP) dropped dramatically. Moreover, the CLPs inhibited biofilm formation in several other strains of pathogenic bacteria without inducing cell death. This study indicates that CLPs from Bacillus sp. 176 show promise as antimicrobial lead compounds targeting bacterial motility and biofilm formation with a low potential for eliciting antibiotic resistance.
KeywordLipopeptide Pumilacidin Vibrio Alginolyticus Motility Antibiotic Antimicrobial Antibiofilm
DOI10.1128/AEM.00450-17
Indexed BySCI
Language英语
WOS IDWOS:000402386500018
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/137093
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xiu, Pengyuan,Liu, Rui,Zhang, Dechao,et al. Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(12).
APA Xiu, Pengyuan,Liu, Rui,Zhang, Dechao,&Sun, Chaomin.(2017).Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(12).
MLA Xiu, Pengyuan,et al."Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.12(2017).
Files in This Item:
File Name/Size DocType Version Access License
Pumilacidin-Like Lip(2690KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xiu, Pengyuan]'s Articles
[Liu, Rui]'s Articles
[Zhang, Dechao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xiu, Pengyuan]'s Articles
[Liu, Rui]'s Articles
[Zhang, Dechao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xiu, Pengyuan]'s Articles
[Liu, Rui]'s Articles
[Zhang, Dechao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.