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Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus
Xiu, Pengyuan1,2,3; Liu, Rui1,2; Zhang, Dechao4; Sun, Chaomin1,2
2017-06-01
发表期刊APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷号83期号:12
文章类型Article
摘要Bacterial motility is a crucial factor during the invasion and colonization processes of pathogens, which makes it an attractive therapeutic drug target. Here, we isolated a marine bacterium (Vibrio alginolyticus strain 178) from a seamount in the tropical West Pacific that exhibits vigorous motility on agar plates and severe pathogenicity to zebrafish. We found that V. alginolyticus 178 motility was significantly suppressed by another marine bacterium, Bacillus sp. strain 176, isolated from the same niche. We isolated, purified, and characterized two different cyclic lipopeptides (CLPs) from Bacillus sp. 176 using high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance spectroscopy. The two related CLPs have a pumilacidin-like structure and were both effective inhibitors of V. alginolyticus 178 motility. The CLPs differ by only one methylene group in their fatty acid chains. In addition to motility suppression, the CLPs also induced cell aggregation in the medium and reduced adherence of V. alginolyticus 178 to glass substrates. Notably, upon CLP treatment, the expression levels of two V. alginolyticus flagellar assembly genes (flgA and flgP) dropped dramatically. Moreover, the CLPs inhibited biofilm formation in several other strains of pathogenic bacteria without inducing cell death. This study indicates that CLPs from Bacillus sp. 176 show promise as antimicrobial lead compounds targeting bacterial motility and biofilm formation with a low potential for eliciting antibiotic resistance.
关键词Lipopeptide Pumilacidin Vibrio Alginolyticus Motility Antibiotic Antimicrobial Antibiofilm
DOI10.1128/AEM.00450-17
收录类别SCI
语种英语
WOS记录号WOS:000402386500018
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/137093
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao, Peoples R China
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Xiu, Pengyuan,Liu, Rui,Zhang, Dechao,et al. Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(12).
APA Xiu, Pengyuan,Liu, Rui,Zhang, Dechao,&Sun, Chaomin.(2017).Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(12).
MLA Xiu, Pengyuan,et al."Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp Strain 176 Suppress the Motility of Vibrio alginolyticus".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.12(2017).
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