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Significant association of cypl9a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus
Fan, Zhaofei1,2,3; Zou, Yuxia1,2; Jiao, Shuang1,2; Tan, Xungang1,2; Wu, Zhihao1,2; Liang, Dongdong1,2,3; Zhang, Peijun1,2; You, Feng1,2
2017-06-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
Volume208Pages:70-79
SubtypeArticle
AbstractThe sex ratio of olive flounder Paralichtilys olivaceus is sensitive to temperature or exogenous hormone exposures in the period of gonadal differentiation. Among sex-related genes, cypl9a, encoding cytochrome P450 aromatase, exhibits significant sex-dimorphic expression pattern and plays an important role in fish gonadal differentiation and development. The present study investigated the expression levels and promoter methylation dynamics of cypl9a and its regulators (nr5a2 and nr0b1), and sex-steroid hormone levels during flounder gonadal differentiation under the treatments of high temperature and estradio1-17 beta (E2). The results showed that levels of flounder cypl9a expression and estradio1-17 beta were repressed by high temperature treatment during this period. The up-regulation of nr5a2 by E2 treatment may be related to the all-female formation, and up-regulation of nrObl by high temperature treatment may be associated with masculinization. Co-transfection assay indicated that nr5a2 and nrObl were antagonist regulators of cypl9a. Furthermore, cypl9a promoter exhibited significant demethylation phenomenon at early stage of ovarian differentiation. While, high temperature could repress the demethylation process, resulting in hypermethylation maintenance in cypl9a promoter. The hypermethylation promoter was able to suppress cypl9a expression by blocking the nr5a2-mediated transactivation activity in vitro. The DNA methylation of epigenetic modification in cypl9a promoter might be the vital way linking environmental factors and gonadal differentiation in flounder. (C) 2017 Published by Elsevier Inc.
KeywordOlive Flounder Paralichthys Olivaceus Cypl9a And Its Regulators Gene Expression Dna Methylation Gonadal Differentiation
DOI10.1016/j.cbpa.2017.02.017
Indexed BySCI
Language英语
WOS IDWOS:000401877100008
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/137085
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Fan, Zhaofei,Zou, Yuxia,Jiao, Shuang,et al. Significant association of cypl9a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2017,208:70-79.
APA Fan, Zhaofei.,Zou, Yuxia.,Jiao, Shuang.,Tan, Xungang.,Wu, Zhihao.,...&You, Feng.(2017).Significant association of cypl9a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,208,70-79.
MLA Fan, Zhaofei,et al."Significant association of cypl9a promoter methylation with environmental factors and gonadal differentiation in olive flounder Paralichthys olivaceus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 208(2017):70-79.
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