IOCAS-IR  > 实验海洋生物学重点实验室
A shell-formation related carbonic anhydrase in Crassostrea gigas modulates intracellular calcium against CO2 exposure: Implication for impacts of ocean acidification on mollusk calcification
Wang, Xiudan1,3; Wang, Mengqiang1; Jia, Zhihao1,3; Song, Xiaorui2; Wang, Lingling2; Song, Linsheng2
2017-08-01
发表期刊AQUATIC TOXICOLOGY
卷号189页码:216-228
文章类型Article
摘要Ocean acidification (OA) could decrease the shells and skeletons formation of mollusk by reducing the availability of carbonate ions at calcification sites. Carbonic anhydrases (CAs) convert CO2 to HCO3- and play important roles in biomineralization process from invertebrate to vertebrate. In the present study, a CA (designated as CgCA) was identified and characterized in Pacific oyster C. gigas. The cDNA of CgCA was of 927 bp encoding a predicted polypeptide of 308 amino acids with a signal peptide and a CA catalytic function domain. The mRNA transcripts of CgCA were constitutively expressed in all tested tissues with the highest levels in mantle and hemocytes. During the early development period, the mRNA transcripts of CgCA could be detected in all the stages with the highest level in D-veliger larvae. Elevated CO2 increased the mRNA transcripts of CgCA in muscle, mantle, hepatopancreas, gill and hemocytes significantly (p < 0.05) and induced the translocation of CgCA in hemocytes and mantle. Moreover, elevated CO2 also caused the decrease of intracellular Ca2+ in hemocytes (p < 0.05). The inhibition of CA by acetazolamide and suppression of CgCA gene via RNA interference could increase the intracellular Ca2+ in hemocytes (p < 0.05). Besides, the decrease of intracellular Ca2+ content caused by Ca2+ reagent ionomycin could affect localization of CgCA in mantle tissue. The results indicated CgCA played essential roles in calcification and elevated CO2 accelerated the mutual modulation between calcium and CgCA, implying reduced calcification rate and dissolved shells under OA.
关键词Ocean Acidification Carbonic Anhydrase Calcification Crassostrea Gigas Co2 Exposure
DOI10.1016/j.aquatox.2017.06.009
收录类别SCI
语种英语
WOS记录号WOS:000405765300024
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/137070
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位实验海洋生物学重点实验室
推荐引用方式
GB/T 7714
Wang, Xiudan,Wang, Mengqiang,Jia, Zhihao,et al. A shell-formation related carbonic anhydrase in Crassostrea gigas modulates intracellular calcium against CO2 exposure: Implication for impacts of ocean acidification on mollusk calcification[J]. AQUATIC TOXICOLOGY,2017,189:216-228.
APA Wang, Xiudan,Wang, Mengqiang,Jia, Zhihao,Song, Xiaorui,Wang, Lingling,&Song, Linsheng.(2017).A shell-formation related carbonic anhydrase in Crassostrea gigas modulates intracellular calcium against CO2 exposure: Implication for impacts of ocean acidification on mollusk calcification.AQUATIC TOXICOLOGY,189,216-228.
MLA Wang, Xiudan,et al."A shell-formation related carbonic anhydrase in Crassostrea gigas modulates intracellular calcium against CO2 exposure: Implication for impacts of ocean acidification on mollusk calcification".AQUATIC TOXICOLOGY 189(2017):216-228.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
A shell-formation re(2567KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Xiudan]的文章
[Wang, Mengqiang]的文章
[Jia, Zhihao]的文章
百度学术
百度学术中相似的文章
[Wang, Xiudan]的文章
[Wang, Mengqiang]的文章
[Jia, Zhihao]的文章
必应学术
必应学术中相似的文章
[Wang, Xiudan]的文章
[Wang, Mengqiang]的文章
[Jia, Zhihao]的文章
相关权益政策
暂无数据
收藏/分享
文件名: A shell-formation related carbonic anhydrase in Crassostrea gigas modulates intracellular calcium against CO2 exposure.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。