| Proteomic analysis in kidneys of Atlantic salmon infected with Aeromonas salmonicida by iTRAQ | |
Liu, Peng-fei1,2; Du, Yishuai1; Meng, Lingjie1,2 ; Li, Xian1; Liu, Ying1,3
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| 2017-07-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 卷号 | 72页码:140-153 |
| 文章类型 | Article |
| 摘要 | Aeromonas salmonicida is a major etiologic agent which induces furunculosis and is globally harmful in salmonid and turbot cultures, especially in Atlantic salmon (Salmo salar) farming. In order to improve knowledge of its poorly understood pathogenesis, we utilized high-throughput proteomics to display differentially expressed proteins in the kidney of Atlantic salmon challenged with high and low infection dose of A. salmonicida at 7 and 14 days. In quantitative proteomic assays, isobaric tags for relative and absolute quantitation (iTRAQ) combined with 2D LC-MS/MS is emerging as a powerful methodology in the search for disease-specific targets and biomarkers. In this study, 4009 distinct proteins (unused >= 1.3, which is a confidence >= 95%) were identified in three two-dimensional LC/MS/MS analyses. Then we chose 140 proteins (fold change ratio >= 1.5 and P < 0.01) combined with protein protein interaction analysis to ultimately obtain 39 proteins in network which could be considered as potential biomarkers for Atlantic salmon immune responses. Nine significant differentially expressed proteins were consistent with those at the proteomic level used to validate genes at the transcriptomic level by qPCR. Collectively, these data was first reported using an iTRAQ approach to provide additional elements for consideration in the pathophysiology of A. salmonicida and pave the way to resolve the influence of this disease in Atlantic salmon. (C) 2017 Elsevier Ltd. All rights reserved. |
| 关键词 | Proteomics Differential Expression Analysis Itraq Function Annotation Analysis Function Enrichment Analysis |
| DOI | 10.1016/j.dci.2017.02.013 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000400038100016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/137048 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Dalian Ocean Univ, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. Proteomic analysis in kidneys of Atlantic salmon infected with Aeromonas salmonicida by iTRAQ[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,72:140-153. |
| APA | Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,Li, Xian,&Liu, Ying.(2017).Proteomic analysis in kidneys of Atlantic salmon infected with Aeromonas salmonicida by iTRAQ.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,72,140-153. |
| MLA | Liu, Peng-fei,et al."Proteomic analysis in kidneys of Atlantic salmon infected with Aeromonas salmonicida by iTRAQ".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 72(2017):140-153. |
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| Proteomic analysis i（4074KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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