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Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay
Ge, Baosheng1; Lin, Xiaojun1; Chen, Yao1; Wang, Xiangfa1; Chen, Huaxin2; Jiang, Peng2; Huang, Fang1
2017-07-01
发表期刊PROCESS BIOCHEMISTRY
卷号58页码:306-312
文章类型Article
摘要The development of fluorescent tools with desired fluorescence and efficient targeting is of great importance in the high-throughput immunoassay. Here we report combinational biosynthesis of new dual-functional streptavidin-phycobiliproteins (SA-PBPs) in Escherichia coli by fusing streptavidin with phycobiliproteins. These recombinant proteins can achieve a purity over 95% after one-step purification, and their maximum absorption and fluorescence emission wavelength are at 556 nm and 568 nm for SA-PCA-PEB (streptavidin-phycocyanin a subunit-phycoerythrin), and 624 nm and 646 nm for SA-PCA-PCB (streptavidin-phycocyanin a subunit-phycocyanobilin), respectively. The potential application of these dual-functional SA-PBPs in immunoassay was evaluated using "sandwich" ELISA method for detection of two biomarkers of liver cancers, i.e. alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA). As a result, both SA-PCA-PCB and SA-PCA-PEB showed a good linear function with AFP & CEA within 0-50 ng/ml concentrations. Their limits,of detection (LODs) for AFP and CEA were 0.25 ng/mL and 0.28 ng/ml using SA-PCA-PEB, and 1.01 ng/ml and 1.12 ng/ml using SA-PCA-PCB respectively. These results indicate that these novel dual-functional SA-PBPs are useful tools for a wide variety of immunoassay, and may have the advantages in their higher expandability and compatibility with existing and future immunoassay technologies.
关键词Combinational Biosynthesis Streptavidin-phycobiliproteins Immunoassay Biomarkers
DOI10.1016/j.procbio.2017.05.006
收录类别SCI
语种英语
WOS记录号WOS:000404710700041
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/137027
专题实验海洋生物学重点实验室
作者单位1.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Ge, Baosheng,Lin, Xiaojun,Chen, Yao,et al. Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay[J]. PROCESS BIOCHEMISTRY,2017,58:306-312.
APA Ge, Baosheng.,Lin, Xiaojun.,Chen, Yao.,Wang, Xiangfa.,Chen, Huaxin.,...&Huang, Fang.(2017).Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay.PROCESS BIOCHEMISTRY,58,306-312.
MLA Ge, Baosheng,et al."Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay".PROCESS BIOCHEMISTRY 58(2017):306-312.
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