IOCAS-IR  > 实验海洋生物学重点实验室
Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay
Ge, Baosheng1; Lin, Xiaojun1; Chen, Yao1; Wang, Xiangfa1; Chen, Huaxin2; Jiang, Peng2; Huang, Fang1
2017-07-01
Source PublicationPROCESS BIOCHEMISTRY
Volume58Pages:306-312
SubtypeArticle
AbstractThe development of fluorescent tools with desired fluorescence and efficient targeting is of great importance in the high-throughput immunoassay. Here we report combinational biosynthesis of new dual-functional streptavidin-phycobiliproteins (SA-PBPs) in Escherichia coli by fusing streptavidin with phycobiliproteins. These recombinant proteins can achieve a purity over 95% after one-step purification, and their maximum absorption and fluorescence emission wavelength are at 556 nm and 568 nm for SA-PCA-PEB (streptavidin-phycocyanin a subunit-phycoerythrin), and 624 nm and 646 nm for SA-PCA-PCB (streptavidin-phycocyanin a subunit-phycocyanobilin), respectively. The potential application of these dual-functional SA-PBPs in immunoassay was evaluated using "sandwich" ELISA method for detection of two biomarkers of liver cancers, i.e. alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA). As a result, both SA-PCA-PCB and SA-PCA-PEB showed a good linear function with AFP & CEA within 0-50 ng/ml concentrations. Their limits,of detection (LODs) for AFP and CEA were 0.25 ng/mL and 0.28 ng/ml using SA-PCA-PEB, and 1.01 ng/ml and 1.12 ng/ml using SA-PCA-PCB respectively. These results indicate that these novel dual-functional SA-PBPs are useful tools for a wide variety of immunoassay, and may have the advantages in their higher expandability and compatibility with existing and future immunoassay technologies.
KeywordCombinational Biosynthesis Streptavidin-phycobiliproteins Immunoassay Biomarkers
DOI10.1016/j.procbio.2017.05.006
Indexed BySCI
Language英语
WOS IDWOS:000404710700041
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/137027
Collection实验海洋生物学重点实验室
Affiliation1.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Ge, Baosheng,Lin, Xiaojun,Chen, Yao,et al. Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay[J]. PROCESS BIOCHEMISTRY,2017,58:306-312.
APA Ge, Baosheng.,Lin, Xiaojun.,Chen, Yao.,Wang, Xiangfa.,Chen, Huaxin.,...&Huang, Fang.(2017).Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay.PROCESS BIOCHEMISTRY,58,306-312.
MLA Ge, Baosheng,et al."Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay".PROCESS BIOCHEMISTRY 58(2017):306-312.
Files in This Item:
File Name/Size DocType Version Access License
Combinational biosyn(615KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ge, Baosheng]'s Articles
[Lin, Xiaojun]'s Articles
[Chen, Yao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ge, Baosheng]'s Articles
[Lin, Xiaojun]'s Articles
[Chen, Yao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ge, Baosheng]'s Articles
[Lin, Xiaojun]'s Articles
[Chen, Yao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Combinational biosynthesis of dual-functional streptavidin-phycobiliproteins for high-throughput-compatible immunoassay.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.