中国科学院海洋研究所机构知识库
Advanced  
IOCAS-IR  > 实验海洋生物学重点实验室  > 期刊论文
题名: Mining and identification of SNP markers associated with growth traits in the clam Meretrix meretrix
作者: Dai, Ping1,2; Wang, Hongxia2; Liu, Baozhong2,3
刊名: AQUACULTURE INTERNATIONAL
出版日期: 2017-06-01
卷号: 25, 期号:3, 页码:1185-1196
关键词: GMD ; Growth ; Marker-trait association ; Meretrix meretrix ; SNPs
DOI: 10.1007/s10499-016-0106-x
文章类型: Article
英文摘要: Selecting fast-growing strains is the most common objective of genetic breeding programs. In this study, we sought to mine the growth-associated single nucleotide polymorphisms (SNPs) in the commercially cultured clam Meretrix meretrix by candidate gene association analysis. Fifty potentially growth-associated contigs based on their functional annotation were chosen as candidates from our EST database, and 240 SNPs were predicted from these contigs. Seventy-one predicted SNPs and five novel SNPs were identified by pooled DNA sequencing in both a fast-growing population (09SPSB) and a control population (09G(3)SB). Among these SNPs, 24 were also identified in an independent population (CP). Through multiplex SNaPshot genotyping, 16 of the 24 SNPs were used in the marker-trait association analysis between 09SPSB and 09G(3)SB. The results showed that seven SNPs in five contigs were significantly associated with clam shell length (P < 0.05). With bidirectional selective genotyping in the CP, three of the seven growth-associated SNPs were further confirmed. Annotations of these SNP-containing contigs provided some clues of these genes functioning in growth. After a haplotype association analysis, we found four haplotypes with significantly different frequencies between 09SPSB and 09G(3)SB (P < 0.001). Through a generalized multifactor dimensionality reduction (GMDR) analysis, we found several gene-gene interaction models, of which the two-factor model (SNPg5/SNPg16) was the best one. These obtained markers will be useful in further quantitative trait loci identification and marker-assisted selection in M. meretrix.
收录类别: SCI
语种: 英语
WOS记录号: WOS:000401852200012
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.qdio.ac.cn/handle/337002/136876
Appears in Collections:实验海洋生物学重点实验室_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Mining and identification of SNP markers associated with growth traits in the clam Meretrix meretrix.pdf(410KB)期刊论文出版稿开放获取View 联系获取全文

作者单位: 1.Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China

Recommended Citation:
Dai, Ping,Wang, Hongxia,Liu, Baozhong. Mining and identification of SNP markers associated with growth traits in the clam Meretrix meretrix[J]. AQUACULTURE INTERNATIONAL,2017,25(3):1185-1196.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Dai, Ping]'s Articles
[Wang, Hongxia]'s Articles
[Liu, Baozhong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Dai, Ping]‘s Articles
[Wang, Hongxia]‘s Articles
[Liu, Baozhong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Mining and identification of SNP markers associated with growth traits in the clam Meretrix meretrix.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院海洋研究所 - Feedback
Powered by CSpace