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iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus
Sun, Lina1; Xu, Dongxue3; Xu, Qinzeng4; Sun, Jingchun1; Xing, Lili1,2; Zhang, Libin1; Yang, Hongsheng1
2017-06-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
Volume22Pages:39-49
SubtypeArticle
AbstractSea cucumbers have a striking capacity to regenerate most of their viscera after evisceration, which has drawn the interest of many researchers. In this study, the isobaric tag for relative and absolute quantitation (iTRAQ) was utilized to investigate protein abundance changes during intestine regeneration in sea cucumbers. A total of 4073 proteins were identified, and 2321 proteins exhibited significantly differential expressions, with 1100 upregulated and 1221 downregulated proteins. Our results suggest that intestine regeneration constitutes a complex life activity regulated by the cooperation of various biological processes, including cytoskeletal changes, extracellular matrix (ECM) remodeling and ECM-receptor interactions, protein synthesis, signal recognition and transduction, energy production and conversion, and substance transport and metabolism. Additionally, real-time PCR showed mRNA expression of differentially expressed genes correlated positively with their protein levels. Our results provided a basis for studying the regulatory mechanisms associated with sea cucumber regeneration. (C) 2017 Elsevier Inc All rights reserved.
KeywordSea Cucumber Intestine Regeneration Itraq Proteomics
DOI10.1016/j.cbd.2017.02.004
Indexed BySCI
Language英语
WOS IDWOS:000402220500005
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136873
Collection海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Peoples R China
4.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Ecol & Environm Sci & Engn, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Lina,Xu, Dongxue,Xu, Qinzeng,et al. iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2017,22:39-49.
APA Sun, Lina.,Xu, Dongxue.,Xu, Qinzeng.,Sun, Jingchun.,Xing, Lili.,...&Yang, Hongsheng.(2017).iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,22,39-49.
MLA Sun, Lina,et al."iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 22(2017):39-49.
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