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The response and osmotic pressure regulation mechanism of Haliotis discus hannai (Mollusca, Gastropoda) to sudden salinity changes
Gao, Xiaolong1,2; Li, Yong1; Li, Xian1; Wu, Fucun1; Song, Changbin3; Liu, Ying2
2017-07-01
Source PublicationHYDROBIOLOGIA
Volume795Issue:1Pages:181-198
SubtypeArticle
AbstractSalinity is one of the critical ecological factors which will impact the growth and development of marine shellfish. With the rapid expansion of aquaculture area for Haliotis discus hannai, the frequent summer rainstorms in South China, the influx of freshwater, or the strong volatility of seawater in coastal areas, inner bays have placed abalone into the dynamic environment where the salinity is changing drastically. This work examined the effects of sudden salinity changes on abalone's survival, osmotic pressure regulation, energy metabolism, and related gene expression by simulating the salinity changes of water for breeding H. discus hannai caused by heavy storm. The salinity was gradually reduced from 30 to 20, then kept at 20 for 48 h, followed by gradual increase to 30, and was kept at 30 for 48 h. Samples were taken at 6, 12, 36, 60, 66, 72, 96, and 120 h after the start of the experiment, respectively. Results showed that the survival rate of abalone at 120 h was significantly lower than that at any other time except at 96 h (P < 0.05). With the decrease and increase of salinity, the hemolymph osmotic pressure and the concentration of Na+, K+, Ca2+, Cl- in the hemolymph also followed the same trend, while the concentration of hemocyanin, total soluble protein, taurine, and free amino acids showed an inverse trend. The activity of Na+/K+-ATPase also increased then declined with salinity changes. Except at 0, 6, and 12 h, the activity of Na+/K+-ATPase in the salinity-changing group was significantly higher than that in the control group (P < 0.05). In the salinity-changing group, the activity of pyruvate kinase, succinate dehydrogenase, and malate dehydrogenase reached a maximum at 72 h, but no significant difference was found at the end of the experiment compared to the control group (P > 0.05). The expression levels of catalase, thioredoxin peroxidase, sigma-glutathione-s-transferase, and Mu-glutathione-s-transferase significantly rose with the salinity changes, and were significantly higher than that in the control group up to the end of the experiment (P < 0.05). As sudden salinity changes may cause some abalone deaths, the enhanced activity of related enzymes and the increase of gene expression levels might be one of the effective methods for an organism to respond to salinity stress and regulate osmotic pressure.
KeywordHaliotis Discus Hannai Ino Salinity Osmotic Pressure Physiological Metabolism Real-time Quantitative Pcr
DOI10.1007/s10750-017-3129-z
Indexed BySCI
Language英语
WOS IDWOS:000401917000013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/136872
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian 116023, Liaoning Provin, Peoples R China
3.Chinese Acad Sci, Inst Semicond, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Gao, Xiaolong,Li, Yong,Li, Xian,et al. The response and osmotic pressure regulation mechanism of Haliotis discus hannai (Mollusca, Gastropoda) to sudden salinity changes[J]. HYDROBIOLOGIA,2017,795(1):181-198.
APA Gao, Xiaolong,Li, Yong,Li, Xian,Wu, Fucun,Song, Changbin,&Liu, Ying.(2017).The response and osmotic pressure regulation mechanism of Haliotis discus hannai (Mollusca, Gastropoda) to sudden salinity changes.HYDROBIOLOGIA,795(1),181-198.
MLA Gao, Xiaolong,et al."The response and osmotic pressure regulation mechanism of Haliotis discus hannai (Mollusca, Gastropoda) to sudden salinity changes".HYDROBIOLOGIA 795.1(2017):181-198.
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