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Two spliced isoforms of the sex-determination gene fruitless in the Chinese mitten crab Eriocheir sinensis
Li, Peiyao1,3; Liu, Yuan1; Luo, Danli1; Song, Chengwen1; Cui, Zhaoxia1,2
2017-06-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume208Pages:75-83
SubtypeArticle
AbstractThe fruitless (fru) gene plays an important role in the sex determination pathway and courtship behavior of Drosophila melanogaster. In the present study, two fru isoforms (Esfru1 and Esfru2) were identified from the Chinese mitten crab Eriocheir sinensis. Sequence analysis showed that Esfru1 and Esfru2 were encoded by the same genomic locus and generated by alternative splicing of pre-mRNA. Esfru1 had all introns completely spliced out, while Esfru2 had a longer exon1 with an additional 78 bp sequence. They both contained a conserved BTB domain which was also found in D. melanogaster Fru isoforms. Analysis on temporal expression profiles of Esfrul-2 (a common region of Esfru1 and Esfru2) and Esfru2 (a specific region of Esfru2) showed that they expressed in similar patterns during embryonic development but in different patterns during larval development. The expression of Esfrul-2 decreased gradually from zoea III stage, however, Esfru2 increased from zoea IV stage and reached the peak at megalopa stage. Expression distribution in tissues and in situ hybridization analysis revealed that they showed sexually dimorphic expression in gonads, hepatopancreas and brains. Esfrul-2 showed significantly higher expression in female gonads and hepatopancreas than in males, but highly expressed in male brains than that in females. Interestingly, Esfru2 was displayed in a male-specific manner in each tissue, especially in gonads and hepatopancreas. Our results indicate that Esfru1 might be involved in both sexual brain neuronal structure development and related to female-specific character development. Esfru2 may participate in male-specific character development. This is the first report that characterizes two spliced variants of fru in crustaceans and provides basic information for further functional studies of the crab sex-determination mechanism.
KeywordEriocheir Sinensis Fruitless Gene Sex Determination Embryonic And Larval Development Sexually Dimorphic Expression
DOI10.1016/j.cbpb.2017.04.008
Indexed BySCI
Language英语
WOS IDWOS:000402220900009
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136842
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
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Li, Peiyao,Liu, Yuan,Luo, Danli,et al. Two spliced isoforms of the sex-determination gene fruitless in the Chinese mitten crab Eriocheir sinensis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2017,208:75-83.
APA Li, Peiyao,Liu, Yuan,Luo, Danli,Song, Chengwen,&Cui, Zhaoxia.(2017).Two spliced isoforms of the sex-determination gene fruitless in the Chinese mitten crab Eriocheir sinensis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,208,75-83.
MLA Li, Peiyao,et al."Two spliced isoforms of the sex-determination gene fruitless in the Chinese mitten crab Eriocheir sinensis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 208(2017):75-83.
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