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Identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn Exopalaemon carinicauda
Lv, Xinjia1,2; Li, Shihao1,3; Liu, Fei1; Li, Fuhua1,3,4; Xiang, Jianhai1,3
2017-05-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume70Pages:128-134
SubtypeArticle
AbstractAnti-lipopolysaccharide factor is a kind of antimicrobial peptide (AMP) with broad-spectrum activities against bacteria, virus, and fungi in crustacean. Different isoforms of ALFs showed different activities to virus or bacteria. Therefore, discovery of more ALFs will provide new insights into drug development and disease control. Here we reported an ALF gene, EcALF1, isolated from the ridgetail prawn Exopalaemon carinicauda. Its transcripts was mainly detected in hemocytes. Silencing of EcALF1 caused a lesion of hepatopancreas and finally led to death of the prawn. In vivo bacteria detection to the hepatopancreas showed that the colonies of thiosulphate citrate bile salts (TCBS) cultured bacteria in EcALF1-silenced prawn increased dramatically. We identified the dominant bacteria cultured in TCBS and found that Vibrio alginolyticus and Vibrio parahaemolyticus were dominant bacteria which were pathogenic species to prawn. We further synthesized the functional domain, LPS binding domain (LBD) of EcALF1, and its antibacterial and antiviral activities were detected. The synthetic EcLBD1 peptide showed very strong antibacterial activity against Micro coccus luteus and V. alginolyticus. Pre-incubation of WSSV with EcLBD1 peptide reduced the pathogenicity of WSSV infection to the prawn. The present data showed the important roles of an ALF gene in modulating the in vivo bacterial proliferation in E. carinicauda and provided new insight in disease control in aquaculture. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordAnti-lipopolysaccharide Factor Rna Interference Bacterial Modulation Antibacterial Antiviral
DOI10.1016/j.dci.2017.01.010
Indexed BySCI
Language英语
WOS IDWOS:000394635500014
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136796
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
4.Natl & Local Joint Engn Lab Ecol Mariculture, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
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Lv, Xinjia,Li, Shihao,Liu, Fei,et al. Identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn Exopalaemon carinicauda[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,70:128-134.
APA Lv, Xinjia,Li, Shihao,Liu, Fei,Li, Fuhua,&Xiang, Jianhai.(2017).Identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn Exopalaemon carinicauda.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,70,128-134.
MLA Lv, Xinjia,et al."Identification and function analysis of an anti-lipopolysaccharide factor from the ridgetail prawn Exopalaemon carinicauda".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 70(2017):128-134.
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