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The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response
Liu, Rui1; Cheng, Qi3; Wang, Xiudan1,4; Chen, Hao1,4; Wang, Weilin1,4; Zhang, Huan1; Wang, Lingling2; Song, Linsheng2
2017-02-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume61Pages:68-78
SubtypeArticle
AbstractB-cell translocation gene 1 (BTG1) is a member of the anti-proliferative gene family, which plays important roles in regulation of cell cycle. In the present study, a B-cell translocation gene 1 molecule homologue (designed CgBTG1) are identified and characterized in oyster Crassostrea gigas. CgBTG1 contains a conserved BTG domain with Box A and Box B motifs, and it shares high similarities with both BTG1 and BTG2 proteins in vertebrates. CgBTG1 mRNA is predominantly expressed in hemocytes, and its expression level in hemocytes is significantly up-regulated at 6 h (5.40-fold, p < 0.01) post Vibrio splendidus stimulation. The apoptosis rate of oyster hemocytes is significantly decreased (p < 0.05) after CgBTG1 interfered by dsRNA (dsCgBTG1). This is indicated that CgBTG1 participated in the regulation of oyster hemocytes apoptosis. Furthermore, CgBTG1 could also induce the apoptosis of cancer cells (HeLa, A549 and BEL7402) in vitro. Compared with normal oysters, both vessel-like structures and muscle fibers in CgBTG1 interfered oysters are severely damaged after V. splendidus challenge in paraffin section, considering that CgBTG1 possessed an analogous feature of angiogenesis for maintenance of vessel-like structures in adductor muscle of oyster. The results suggests that CgBTG1 is a multi-functional molecule involved in the immune response of C. gigas against pathogen infection, which provides more clues for intensive studies of BTG family proteins in invertebrates. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordB-cell Translocation Gene 1 Crassostrea Gigas Anti-proliferation Apoptosis Immune Response Pathogen Infection
DOI10.1016/j.fsi.2016.12.005
Indexed BySCI
Language英语
WOS IDWOS:000394200400008
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136789
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
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Liu, Rui,Cheng, Qi,Wang, Xiudan,et al. The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2017,61:68-78.
APA Liu, Rui.,Cheng, Qi.,Wang, Xiudan.,Chen, Hao.,Wang, Weilin.,...&Song, Linsheng.(2017).The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response.FISH & SHELLFISH IMMUNOLOGY,61,68-78.
MLA Liu, Rui,et al."The B-cell translocation gene 1 (CgBTG1) identified in oyster Crassostrea gigas exhibit multiple functions in immune response".FISH & SHELLFISH IMMUNOLOGY 61(2017):68-78.
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