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Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus
Liu Meng1,2; Liu Yuan2; Hui Min2; Song Chengwen2; Cui Zhaoxia2,3,4
2017-03-01
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume35Issue:2Pages:235-243
SubtypeArticle
AbstractClip domain serine proteases (cSPs) and their homologs (SPHs) play an important role in various biological processes that are essential components of extracellular signaling cascades, especially in the innate immune responses of invertebrates. Here, polymorphisms of PtcSP and PtSPH from the swimming crab Portunus trituberculatus were investigated to explore their association with resistance/susceptibility to Vibrio alginolyticus. Polymorphic loci were identified using Clustal X, and characterized with SPSS 16.0 software, and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by a. 2 test. A total of 109 and 77 single nucleotide polymorphisms (SNPs) were identified in the genomic fragments of PtcSP and PtSPH, respectively. Notably, nearly half of PtSPH polymorphisms were found in the non-coding exon 1. Fourteen SNPs investigated were significantly associated with susceptibility/resistance to V. alginolyticus (P<0.05). Among them, eight SNPs were observed in introns, and one synonymous, four non-synonymous SNPs and one ins-del were found in coding exons. In addition, five simple sequence repeats (SSRs) were detected in intron 3 of PtcSP. Although there was no statistically significant difference of allele frequencies, the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks. After further validation, polymorphisms investigated here might be applied to select potential molecular markers of P. trituberculatus with resistance to V. alginolyticus
KeywordPortunus Trituberculatus Clip Domain Serine Proteinase Serine Proteinase Homolog Polymorphism Susceptibility/resistance
DOI10.1007/s00343-016-5331-7
Indexed BySCI
Language英语
WOS IDWOS:000396021400002
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136778
Collection实验海洋生物学重点实验室
Affiliation1.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
4.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Liu Meng,Liu Yuan,Hui Min,et al. Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(2):235-243.
APA Liu Meng,Liu Yuan,Hui Min,Song Chengwen,&Cui Zhaoxia.(2017).Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(2),235-243.
MLA Liu Meng,et al."Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.2(2017):235-243.
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