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Functional Elucidation of Nemopilema nomurai and Cyanea nozakii Nematocyst Venoms' Lytic Activity Using Mass Spectrometry and Zymography
Yue, Yang1,2; Yu, Huahua1; Li, Rongfeng1; Xing, Ronge1; Liu, Song1; Li, Kecheng1; Wang, Xueqin1; Chen, Xiaolin1; Li, Pengcheng1
2017-02-01
Source PublicationTOXINS
Volume9Issue:2
SubtypeArticle
AbstractBackground: Medusozoans utilize explosively discharging penetrant nematocysts to inject venom into prey. These venoms are composed of highly complex proteins and peptides with extensive bioactivities, as observed in vitro. Diverse enzymatic toxins have been putatively identified in the venom of jellyfish, Nemopilema nomurai and Cyanea nozakii, through examination of their proteomes and transcriptomes. However, functional examination of putative enzymatic components identified in proteomic approaches to elucidate potential bioactivities is critically needed. Methods: In this study, enzymatic toxins were functionally identified using a combined approach consisting of in gel zymography and liquid chromatography tandem mass spectrometry (LC-MS/MS). The potential roles of metalloproteinases and lipases in hemolytic activity were explored using specific inhibitors. Results: Zymography indicated that nematocyst venom possessed protease-, lipase-and hyaluronidase-class activities. Further, proteomic approaches using LC-MS/MS indicated sequence homology of proteolytic bands observed in zymography to extant zinc metalloproteinase-disintegrins and astacin metalloproteinases. Moreover, pre-incubation of the metalloproteinase inhibitor batimastat with N. nomurai nematocyst venom resulted in an approximate 62% reduction of hemolysis compared to venom exposed sheep erythrocytes, suggesting that metalloproteinases contribute to hemolytic activity. Additionally, species within the molecular mass range of 14-18 kDa exhibited both egg yolk and erythrocyte lytic activities in gel overlay assays. Conclusion: For the first time, our findings demonstrate the contribution of jellyfish venom metalloproteinase and suggest the involvement of lipase species to hemolytic activity. Investigations of this relationship will facilitate a better understanding of the constituents and toxicity of jellyfish venom.
KeywordZymography Lc-ms/ms Enzymatic Toxins Hemolysis Jellyfish Metalloproteinase Inhibitors Pla(2) Inhibitors Nemopilema Nomurai Cyanea Nozakii
DOI10.3390/toxins9020047
Indexed BySCI
Language英语
WOS IDWOS:000395450500005
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136742
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
海洋环境工程技术研究发展中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Yue, Yang,Yu, Huahua,Li, Rongfeng,et al. Functional Elucidation of Nemopilema nomurai and Cyanea nozakii Nematocyst Venoms' Lytic Activity Using Mass Spectrometry and Zymography[J]. TOXINS,2017,9(2).
APA Yue, Yang.,Yu, Huahua.,Li, Rongfeng.,Xing, Ronge.,Liu, Song.,...&Li, Pengcheng.(2017).Functional Elucidation of Nemopilema nomurai and Cyanea nozakii Nematocyst Venoms' Lytic Activity Using Mass Spectrometry and Zymography.TOXINS,9(2).
MLA Yue, Yang,et al."Functional Elucidation of Nemopilema nomurai and Cyanea nozakii Nematocyst Venoms' Lytic Activity Using Mass Spectrometry and Zymography".TOXINS 9.2(2017).
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