A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish

doi:10.1016/j.dci.2017.01.021

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	A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish
	Li, Xue-peng1,2,3 ; Sun, Li 1,2
	2017-06-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	71 页码:49-58
文章类型	Article
摘要	Complement factor B (Bf) is a component of the complement system. Following activation of the alternative pathway of the complement system, factor B is cleaved into Ba and Bb fragments. In fish, the Bf of rainbow trout is known to act as a C3 convertase, but the function of the Ba fragment is essentially unknown. In this study, we examined the expression patterns of tongue sole Cynoglossus semilaevis Bf (named CsBf) and the biological activity of the Ba fragment of CsBf (named CsBa). CsBf possesses the conserved domains of Bf and shares 39.9%-56.4% sequence identities With other fish Bf. CsBf expression was high in liver, muscle, and heart, and low in intestine, blood, and kidney. Bacterial infection significantly induced CsBf expression in kidney, spleen, and liver in a time-dependent manner. Recombinant CsBa (rCsBa) exhibited apparent binding capacities to bacteria and tongue sole peripheral blood leukocytes, and binding of rCsBa to bacteria inhibited bacterial growth. When overexpressed in tongue sole, CsBa significantly reduced bacterial dissemination in fish tissues. Together these results indicate for the first time that a fish Ba possesses antibacterial effect as well as immune cell-binding capacity, and thus probably plays a role in host immune defense against bacterial infection. (C) 2017 Elsevier Ltd. All rights reserved.
关键词	Complement Factor b Cynoglossus Semilaevis Antimicrobial Bacterial Infection
DOI	10.1016/j.dci.2017.01.021
收录类别	SCI
语种	英语
WOS记录号	WOS:000397834800006
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136727
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Xue-peng,Sun, Li. A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,71:49-58.
APA	Li, Xue-peng,&Sun, Li.(2017).A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,71,49-58.
MLA	Li, Xue-peng,et al."A teleost complement factor Ba possesses antimicrobial activity and inhibits bacterial infection in fish".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 71(2017):49-58.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
A teleost complement（4555KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览