IOCAS-IR  > 实验海洋生物学重点实验室
Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster
Huan, Pin1; Wang, Hongxia1; Liu, Baozhong1,2
2016-10-01
Source PublicationGENES & GENETIC SYSTEMS
Volume91Issue:5Pages:257-265
SubtypeArticle
AbstractThe early development of mollusks exhibits important characteristics from the developmental and evolutionary perspective. With the increasing number of genome-wide studies, accurate analyses of quantitative gene expression during development are impeded by the lack of validated reference genes. To improve the situation, in this study, we analyzed the expression stability of seven candidate housekeeping genes during early development of the Pacific oyster Crassostrea gigas: actin, glyceraldehyde-3-phosphate dehydrogenase (gapdh), a subunit of elongation factor 1 (elf1 alpha), adp-ribosylation factor 1 (arf1), heterogeneous nuclear ribonucleoprotein q, ubiquitin-conjugating enzyme e2d2 and ribosomal protein s18. We focused on 11 stages from oocyte to D-veliger, which include crucial developmental processes such as axis determination, gastrulation and shell formation. Gene expression stabilities were assessed with the three commonly used programs geNorm, NorniFinder and BestKeeper. Although the results obtained with the three programs varied to some extent, in general, arf1, elf1 alpha and gapdh were highly ranked and actin was poorly ranked. This analysis also indicated that multiple genes should be used for normalization, and we concluded that arf1-elf1 alpha-gapdh should be used as internal references. The findings of this study will help researchers to obtain accurate results in future quantitative gene expression analysis of development in bivalve mollusks.
KeywordCrassostrea Gigas Expression Stability Internal Reference Normalization Real-time Pcr
DOI10.1266/ggs.16-00007
Indexed BySCI
Language英语
WOS IDWOS:000399356100002
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136709
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Huan, Pin,Wang, Hongxia,Liu, Baozhong. Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster[J]. GENES & GENETIC SYSTEMS,2016,91(5):257-265.
APA Huan, Pin,Wang, Hongxia,&Liu, Baozhong.(2016).Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster.GENES & GENETIC SYSTEMS,91(5),257-265.
MLA Huan, Pin,et al."Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster".GENES & GENETIC SYSTEMS 91.5(2016):257-265.
Files in This Item:
File Name/Size DocType Version Access License
Assessment of housek(682KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huan, Pin]'s Articles
[Wang, Hongxia]'s Articles
[Liu, Baozhong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huan, Pin]'s Articles
[Wang, Hongxia]'s Articles
[Liu, Baozhong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huan, Pin]'s Articles
[Wang, Hongxia]'s Articles
[Liu, Baozhong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.