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Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster
Huan, Pin1; Wang, Hongxia1; Liu, Baozhong1,2
2016-10-01
发表期刊GENES & GENETIC SYSTEMS
卷号91期号:5页码:257-265
文章类型Article
摘要The early development of mollusks exhibits important characteristics from the developmental and evolutionary perspective. With the increasing number of genome-wide studies, accurate analyses of quantitative gene expression during development are impeded by the lack of validated reference genes. To improve the situation, in this study, we analyzed the expression stability of seven candidate housekeeping genes during early development of the Pacific oyster Crassostrea gigas: actin, glyceraldehyde-3-phosphate dehydrogenase (gapdh), a subunit of elongation factor 1 (elf1 alpha), adp-ribosylation factor 1 (arf1), heterogeneous nuclear ribonucleoprotein q, ubiquitin-conjugating enzyme e2d2 and ribosomal protein s18. We focused on 11 stages from oocyte to D-veliger, which include crucial developmental processes such as axis determination, gastrulation and shell formation. Gene expression stabilities were assessed with the three commonly used programs geNorm, NorniFinder and BestKeeper. Although the results obtained with the three programs varied to some extent, in general, arf1, elf1 alpha and gapdh were highly ranked and actin was poorly ranked. This analysis also indicated that multiple genes should be used for normalization, and we concluded that arf1-elf1 alpha-gapdh should be used as internal references. The findings of this study will help researchers to obtain accurate results in future quantitative gene expression analysis of development in bivalve mollusks.
关键词Crassostrea Gigas Expression Stability Internal Reference Normalization Real-time Pcr
DOI10.1266/ggs.16-00007
收录类别SCI
语种英语
WOS记录号WOS:000399356100002
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136709
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China
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Huan, Pin,Wang, Hongxia,Liu, Baozhong. Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster[J]. GENES & GENETIC SYSTEMS,2016,91(5):257-265.
APA Huan, Pin,Wang, Hongxia,&Liu, Baozhong.(2016).Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster.GENES & GENETIC SYSTEMS,91(5),257-265.
MLA Huan, Pin,et al."Assessment of housekeeping genes as internal references in quantitative expression analysis during early development of oyster".GENES & GENETIC SYSTEMS 91.5(2016):257-265.
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