IOCAS-IR  > 海洋腐蚀与防护研究发展中心
Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection
Zheng, Laibao1,2; Wan, Yi1; Qi, Peng1; Sun, Yan1; Zhang, Dun1; Yu, Liangmin3,4
2017-05-15
Source PublicationTALANTA
Volume167Pages:600-606
SubtypeArticle
AbstractThe detection of pathogenic bacteria is essential in various fields, such as food safety, water environmental analysis, or clinical diagnosis. Although rapid and selective techniques have been achieved based on the fast and specific binding of recognitions elements and target, the sensitive detection of bacterial pathogens was limited by their low targets-binding efficiency. The three-dimensional (3D) nano-biointerface, compared with the two-dimensional (2D) flat substrate, has a much higher binding capacity, which can offer more reactive sites to bind with bacterial targets, resulting in a great improvement of detection sensitivity. Herein, a lectin functionalized ZnO nanorod (ZnO-NR) array has been fabricated and employed as a 3D nano-biointerface for Escherichia coli (E. con) capture and detection by multivalent binding of concanavalin A (ConA) with polysaccharides on the cellular surface of E. coli. The 3D lectin functionalized ZnO-NR array-based assay shows reasonable detection limit and efficiently expanded linear range (1.0x10(3) to 1.0x10(7) cfu mL(-1)) for pathogen detection. The platform has a potential for further applications and provides an excellent sensitivity approach for detection of pathogenic bacteria.
Keyword3d Nano-interface Zno-nr Arrays Lectin Functionalization Bacterial Detection
DOI10.1016/j.talanta.2017.03.007
Indexed BySCI
Language英语
WOS IDWOS:000399511600075
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136699
Collection海洋腐蚀与防护研究发展中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Ocean Univ China, Key Lab Marine Chem Theory & Technol, Minist Educ, Qingdao 266100, Peoples R China
4.Ocean Univ China, Qingdao Collaborat Innovat Ctr Marine Sci & Techn, Qingdao 266100, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Laibao,Wan, Yi,Qi, Peng,et al. Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection[J]. TALANTA,2017,167:600-606.
APA Zheng, Laibao,Wan, Yi,Qi, Peng,Sun, Yan,Zhang, Dun,&Yu, Liangmin.(2017).Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection.TALANTA,167,600-606.
MLA Zheng, Laibao,et al."Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection".TALANTA 167(2017):600-606.
Files in This Item:
File Name/Size DocType Version Access License
Lectin functionalize(1248KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zheng, Laibao]'s Articles
[Wan, Yi]'s Articles
[Qi, Peng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zheng, Laibao]'s Articles
[Wan, Yi]'s Articles
[Qi, Peng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zheng, Laibao]'s Articles
[Wan, Yi]'s Articles
[Qi, Peng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Lectin functionalized ZnO nanoarrays as a 3D nano-biointerface for bacterial detection.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.