Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis

doi:10.1007/s10695-016-0294-3

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	Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis
	Jiao, Shuang 1,2; Wu, Zhihao 1,2; Tan, Xungang1,2 ; Sui, Yulei1,2,3 ; Wang, Lijuan1,2,3 ; You, Feng 1,2
	2017-04-01
发表期刊	FISH PHYSIOLOGY AND BIOCHEMISTRY
卷号	43 期号:2 页码:385-395
文章类型	Article
摘要	Although chromosome set manipulation techniques including polyploidy induction and gynogentic induction in flatfish are becoming increasingly mature, there exists a poor understanding of their effects on embryonic development. PAX3 plays crucial roles during embryonic myogenesis and neurogenesis. In olive flounder (Paralichthys olivaceus), there are two duplicated pax3 genes (pax3a, pax3b), and both of them are expressed in the brain and muscle regions with some subtle regional differences. We utilized pax3a and pax3b as indicators to preliminarily investigate whether chromosome set manipulation affects embryonic neurogenesis and myogenesis using whole-mount in situ hybridization. In the polyploid induction groups, 94 % of embryos in the triploid induction group had normal pax3a/3b expression patterns; however, 45 % of embryos in the tetraploid induction group showed abnormal pax3a/3b expression patterns from the tailbud formation stage to the hatching stage. Therefore, the artificial induction of triploidy and tetraploidy had a small or a moderate effect on flounder embryonic myogenesis and neurogenesis, respectively. In the gynogenetic induction groups, 87 % of embryos in the meiogynogenetic diploid induction group showed normal pax3a/3b expression patterns. However, almost 100 % of embryos in the gynogenetic haploid induction group and 63 % of embryos in the mitogynogenetic diploid induction group showed abnormal pax3a/3b expression patterns. Therefore, the induction of gynogenetic haploidy and mitogynogenetic diploidy had large effects on flounder embryonic myogenesis and neurogenesis. In conclusion, the differential expression of pax3a and pax3b may provide new insights for consideration of fish chromosome set manipulation.
关键词	Gynogenetic Induction Group Olive Flounder (Paralichthys Olivaceus) Pax3a Pax3b Triploid Induction Group Tetraploid Induction Group
DOI	10.1007/s10695-016-0294-3
收录类别	SCI
语种	英语
WOS记录号	WOS:000399017500008
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136698
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Jiao, Shuang,Wu, Zhihao,Tan, Xungang,et al. Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2017,43(2):385-395.
APA	Jiao, Shuang,Wu, Zhihao,Tan, Xungang,Sui, Yulei,Wang, Lijuan,&You, Feng.(2017).Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis.FISH PHYSIOLOGY AND BIOCHEMISTRY,43(2),385-395.
MLA	Jiao, Shuang,et al."Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis".FISH PHYSIOLOGY AND BIOCHEMISTRY 43.2(2017):385-395.

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