IOCAS-IR  > 实验海洋生物学重点实验室
Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis
Jiao, Shuang1,2; Wu, Zhihao1,2; Tan, Xungang1,2; Sui, Yulei1,2,3; Wang, Lijuan1,2,3; You, Feng1,2
2017-04-01
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
Volume43Issue:2Pages:385-395
SubtypeArticle
AbstractAlthough chromosome set manipulation techniques including polyploidy induction and gynogentic induction in flatfish are becoming increasingly mature, there exists a poor understanding of their effects on embryonic development. PAX3 plays crucial roles during embryonic myogenesis and neurogenesis. In olive flounder (Paralichthys olivaceus), there are two duplicated pax3 genes (pax3a, pax3b), and both of them are expressed in the brain and muscle regions with some subtle regional differences. We utilized pax3a and pax3b as indicators to preliminarily investigate whether chromosome set manipulation affects embryonic neurogenesis and myogenesis using whole-mount in situ hybridization. In the polyploid induction groups, 94 % of embryos in the triploid induction group had normal pax3a/3b expression patterns; however, 45 % of embryos in the tetraploid induction group showed abnormal pax3a/3b expression patterns from the tailbud formation stage to the hatching stage. Therefore, the artificial induction of triploidy and tetraploidy had a small or a moderate effect on flounder embryonic myogenesis and neurogenesis, respectively. In the gynogenetic induction groups, 87 % of embryos in the meiogynogenetic diploid induction group showed normal pax3a/3b expression patterns. However, almost 100 % of embryos in the gynogenetic haploid induction group and 63 % of embryos in the mitogynogenetic diploid induction group showed abnormal pax3a/3b expression patterns. Therefore, the induction of gynogenetic haploidy and mitogynogenetic diploidy had large effects on flounder embryonic myogenesis and neurogenesis. In conclusion, the differential expression of pax3a and pax3b may provide new insights for consideration of fish chromosome set manipulation.
KeywordGynogenetic Induction Group Olive Flounder (Paralichthys Olivaceus) Pax3a Pax3b Triploid Induction Group Tetraploid Induction Group
DOI10.1007/s10695-016-0294-3
Indexed BySCI
Language英语
WOS IDWOS:000399017500008
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136698
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jiao, Shuang,Wu, Zhihao,Tan, Xungang,et al. Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2017,43(2):385-395.
APA Jiao, Shuang,Wu, Zhihao,Tan, Xungang,Sui, Yulei,Wang, Lijuan,&You, Feng.(2017).Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis.FISH PHYSIOLOGY AND BIOCHEMISTRY,43(2),385-395.
MLA Jiao, Shuang,et al."Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis".FISH PHYSIOLOGY AND BIOCHEMISTRY 43.2(2017):385-395.
Files in This Item:
File Name/Size DocType Version Access License
Characterization of (2001KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jiao, Shuang]'s Articles
[Wu, Zhihao]'s Articles
[Tan, Xungang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jiao, Shuang]'s Articles
[Wu, Zhihao]'s Articles
[Tan, Xungang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jiao, Shuang]'s Articles
[Wu, Zhihao]'s Articles
[Tan, Xungang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.