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Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis
Jiao, Shuang1,2; Wu, Zhihao1,2; Tan, Xungang1,2; Sui, Yulei1,2,3; Wang, Lijuan1,2,3; You, Feng1,2
2017-04-01
发表期刊FISH PHYSIOLOGY AND BIOCHEMISTRY
卷号43期号:2页码:385-395
文章类型Article
摘要Although chromosome set manipulation techniques including polyploidy induction and gynogentic induction in flatfish are becoming increasingly mature, there exists a poor understanding of their effects on embryonic development. PAX3 plays crucial roles during embryonic myogenesis and neurogenesis. In olive flounder (Paralichthys olivaceus), there are two duplicated pax3 genes (pax3a, pax3b), and both of them are expressed in the brain and muscle regions with some subtle regional differences. We utilized pax3a and pax3b as indicators to preliminarily investigate whether chromosome set manipulation affects embryonic neurogenesis and myogenesis using whole-mount in situ hybridization. In the polyploid induction groups, 94 % of embryos in the triploid induction group had normal pax3a/3b expression patterns; however, 45 % of embryos in the tetraploid induction group showed abnormal pax3a/3b expression patterns from the tailbud formation stage to the hatching stage. Therefore, the artificial induction of triploidy and tetraploidy had a small or a moderate effect on flounder embryonic myogenesis and neurogenesis, respectively. In the gynogenetic induction groups, 87 % of embryos in the meiogynogenetic diploid induction group showed normal pax3a/3b expression patterns. However, almost 100 % of embryos in the gynogenetic haploid induction group and 63 % of embryos in the mitogynogenetic diploid induction group showed abnormal pax3a/3b expression patterns. Therefore, the induction of gynogenetic haploidy and mitogynogenetic diploidy had large effects on flounder embryonic myogenesis and neurogenesis. In conclusion, the differential expression of pax3a and pax3b may provide new insights for consideration of fish chromosome set manipulation.
关键词Gynogenetic Induction Group Olive Flounder (Paralichthys Olivaceus) Pax3a Pax3b Triploid Induction Group Tetraploid Induction Group
DOI10.1007/s10695-016-0294-3
收录类别SCI
语种英语
WOS记录号WOS:000399017500008
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136698
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Jiao, Shuang,Wu, Zhihao,Tan, Xungang,et al. Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2017,43(2):385-395.
APA Jiao, Shuang,Wu, Zhihao,Tan, Xungang,Sui, Yulei,Wang, Lijuan,&You, Feng.(2017).Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis.FISH PHYSIOLOGY AND BIOCHEMISTRY,43(2),385-395.
MLA Jiao, Shuang,et al."Characterization of pax3a and pax3b genes in artificially induced polyploid and gynogenetic olive flounder (Paralichthys olivaceus) during embryogenesis".FISH PHYSIOLOGY AND BIOCHEMISTRY 43.2(2017):385-395.
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