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A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya
Li, Caiwen1,2; Chen, Tiantian1,2,3
2017-02-01
发表期刊HARMFUL ALGAE
卷号62页码:30-36
文章类型Article
摘要The parasitic dinoflagellate Amoebophrya infects broad range of marine organisms. Particularly, Amoebophrya infections in planktonic dinoflagellates can prevent or delay the formation of algal blooms, and recycle undergrazed planktonic dinoflagellates back to the microbial loop by disrupting host cells. Its ecological significance was gradually recognized along with the discovery of its enormous molecular diversity in oceanic and coastal ecosystems. Thus, we developed a reliable, easily accessible and less time-consuming assay, to detect and assess Amoebophrya infections in planktonic dinoflagellates. The modified hematoxylin and eosin staining assay provided reliable diagnosis of Amoebophrya infection by identifying the characteristic "beehive" of the multinucleate trophonts. After staining, the typical multinucleate "beehive" is evidently distinguishable from the compact nuclei of uninfected host cells. The modified hematoxylin and eosin (H & E) staining assay is easy to use, that can be routinely performed within 3 h (up to 20 samples/batch) using general laboratory equipment, supplies and chemical reagents. The produced slides with agar-embedded dinoflagellate cells can be stored for several months or even years in a dry place without noticeable loss in quality of staining. With suitable calculation, the modified H & E assay can be applied to assess the prevalence of Amoebophrya infection in planktonic dinoflagellates. This efficient and powerful assay will facilitate the investigation on the ecological roles of Amoebophryidae in coastal and oceanic ecosystem. (C) 2016 Elsevier B.V. All rights reserved.
关键词Marine Alveolate Parasite Life Cycle Trophont Prevalence
DOI10.1016/j.hal.2016.12.003
收录类别SCI
语种英语
WOS记录号WOS:000393929800004
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136388
专题海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China
3.Univ Chinese Acad Sci, Beijing 10039, Peoples R China
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Li, Caiwen,Chen, Tiantian. A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya[J]. HARMFUL ALGAE,2017,62:30-36.
APA Li, Caiwen,&Chen, Tiantian.(2017).A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya.HARMFUL ALGAE,62,30-36.
MLA Li, Caiwen,et al."A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya".HARMFUL ALGAE 62(2017):30-36.
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