IOCAS-IR  > 海洋生态与环境科学重点实验室
A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya
Li, Caiwen1,2; Chen, Tiantian1,2,3
2017-02-01
Source PublicationHARMFUL ALGAE
Volume62Pages:30-36
SubtypeArticle
AbstractThe parasitic dinoflagellate Amoebophrya infects broad range of marine organisms. Particularly, Amoebophrya infections in planktonic dinoflagellates can prevent or delay the formation of algal blooms, and recycle undergrazed planktonic dinoflagellates back to the microbial loop by disrupting host cells. Its ecological significance was gradually recognized along with the discovery of its enormous molecular diversity in oceanic and coastal ecosystems. Thus, we developed a reliable, easily accessible and less time-consuming assay, to detect and assess Amoebophrya infections in planktonic dinoflagellates. The modified hematoxylin and eosin staining assay provided reliable diagnosis of Amoebophrya infection by identifying the characteristic "beehive" of the multinucleate trophonts. After staining, the typical multinucleate "beehive" is evidently distinguishable from the compact nuclei of uninfected host cells. The modified hematoxylin and eosin (H & E) staining assay is easy to use, that can be routinely performed within 3 h (up to 20 samples/batch) using general laboratory equipment, supplies and chemical reagents. The produced slides with agar-embedded dinoflagellate cells can be stored for several months or even years in a dry place without noticeable loss in quality of staining. With suitable calculation, the modified H & E assay can be applied to assess the prevalence of Amoebophrya infection in planktonic dinoflagellates. This efficient and powerful assay will facilitate the investigation on the ecological roles of Amoebophryidae in coastal and oceanic ecosystem. (C) 2016 Elsevier B.V. All rights reserved.
KeywordMarine Alveolate Parasite Life Cycle Trophont Prevalence
DOI10.1016/j.hal.2016.12.003
Indexed BySCI
Language英语
WOS IDWOS:000393929800004
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136388
Collection海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China
3.Univ Chinese Acad Sci, Beijing 10039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Caiwen,Chen, Tiantian. A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya[J]. HARMFUL ALGAE,2017,62:30-36.
APA Li, Caiwen,&Chen, Tiantian.(2017).A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya.HARMFUL ALGAE,62,30-36.
MLA Li, Caiwen,et al."A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya".HARMFUL ALGAE 62(2017):30-36.
Files in This Item:
File Name/Size DocType Version Access License
A novel hematoxylin (1195KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Caiwen]'s Articles
[Chen, Tiantian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Caiwen]'s Articles
[Chen, Tiantian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Caiwen]'s Articles
[Chen, Tiantian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A novel hematoxylin and eosin stain assay for detection of the parasitic dinoflagellate Amoebophrya.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.