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Identification and validation of sRNAs in Edwardsiella tarda S08
Sun, Yuying1,2,3; Zhang, Jiquan4; Qin, Lei1; Yan, Cui1; Zhang, Xiaojun1; Liu, Dandan1
Source PublicationPLOS ONE
AbstractBacterial small non-coding RNAs (sRNAs) are known as novel regulators involved in virulence, stress responsibility, and so on. Recently, a lot of new researches have highlighted the critical roles of sRNAs in fine-tune gene regulation in both prokaryotes and eukaryotes. Edwardsiella tarda (E. tarda) is a gram-negative, intracellular pathogen that causes edward-siellosis in fish. Thus far, no sRNA has been reported in E. tarda. The present study represents the first attempt to identify sRNAs in E. tarda S08. Ten sRNAs were validated by RNA sequencing and quantitative PCR (qPCR). ET_sRNA_1 and ET_sRNA_2 were homolous to tmRNA and GcvB, respectively. However, the other candidate sRNAs have not been reported till now. The cellular abundance of 10 validated sRNA was detected by qPCR at different growth phases to monitor their biosynthesis. Nine candidate sRNAs were expressed in the late-stage of exponential growth and stationary stages of growth (36 similar to 60 h). And the expression of the nine sRNAs was growth phase-dependent. But ET_sRNA_10 was almost expressed all the time and reached the highest peak at 48 h. Their targets were predicted by TargetRNA2 and each sRNA target contains some genes that directly or indirectly relate to virulence. These results preliminary showed that sRNAs probably play a regulatory role of virulence in E. tarda.
Indexed BySCI
WOS IDWOS:000396060600003
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Huaihai Inst Technol, Coll Marine Life & Fisheries, Lianyungang, Peoples R China
2.Jiangsu Marine Resources Dev Res Inst, Lianyungang, Peoples R China
3.Huaihai Inst Technol, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Sun, Yuying,Zhang, Jiquan,Qin, Lei,et al. Identification and validation of sRNAs in Edwardsiella tarda S08[J]. PLOS ONE,2017,12(3).
APA Sun, Yuying,Zhang, Jiquan,Qin, Lei,Yan, Cui,Zhang, Xiaojun,&Liu, Dandan.(2017).Identification and validation of sRNAs in Edwardsiella tarda S08.PLOS ONE,12(3).
MLA Sun, Yuying,et al."Identification and validation of sRNAs in Edwardsiella tarda S08".PLOS ONE 12.3(2017).
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