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A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis
He, Shu-wen1; Zhang, Jian2; Li, Ning-qiu3; Zhou, Shun1; Yue, Bin1; Zhang, Min1
2017
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume60Pages:466-473
SubtypeArticle
AbstractTissue factor pathway inhibitor 1 (TFPI-1) is a serine protease inhibitor that inhibits tissue factor (TF)-mediated coagulation. The C-terminal region of TFPI-1 could be cleaved off and proved to be antimicrobial against a broad-spectrum of microorganism. In a previous study, a C-terminal peptide, TC24 (with 24 amino acids), derived from tongue sole (Cynoglossus semilaevis) TFPI-1, was synthesized and found antibacterial against Micrococcus luteus. In the present study, the antibacterial spectrum and the action mode of TC24 was further examined, and its in vivo function was analyzed. Our results showed that TC24 also possesses bactericidal activity against Staphylococcus aureus and Vibrio vulnificus. During its interaction with the target bacterial cells, TC24 destroyed cell membrane integrity, penetrated into the cytoplasm, and induced degradation of genomic DNA and total RNA. In vivo study showed that administration of tongue sole with TC24 before bacterial and viral infection significantly reduced pathogen dissemination and replication in tissues. These results indicated that TC24 is a novel antimicrobial peptide against bacterial and viral pathogens, and that the observed effect of TC24 on bacterial RNA adds new insights to the action mechanism of fish antimicrobial peptides. Moreover, TC24 may play an important role in fighting pathogenic infection in aquaculture. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordAntimicrobial Peptide Tfpi-1 Antibacterial Mechanism Antiviral Teleost
DOI10.1016/j.fsi.2016.11.029
Indexed BySCI
Language英语
WOS IDWOS:000392770000051
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136317
Collection实验海洋生物学重点实验室
Affiliation1.Qingdao Agr Univ, Marine Sci & Engn Coll, 700 Changcheng Rd, Qingdao 266109, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab Aquat Anim Immune Technol, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev,Minist Agr, Guangzhou 510380, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
He, Shu-wen,Zhang, Jian,Li, Ning-qiu,et al. A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis[J]. FISH & SHELLFISH IMMUNOLOGY,2017,60:466-473.
APA He, Shu-wen,Zhang, Jian,Li, Ning-qiu,Zhou, Shun,Yue, Bin,&Zhang, Min.(2017).A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis.FISH & SHELLFISH IMMUNOLOGY,60,466-473.
MLA He, Shu-wen,et al."A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis".FISH & SHELLFISH IMMUNOLOGY 60(2017):466-473.
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