IOCAS-IR  > 实验海洋生物学重点实验室
Gene set based association analyses for the WSSV resistance of Pacific white shrimp Litopenaeus vannamei
Yu, Yang1; Liu, Jingwen1,2; Li, Fuhua1,3; Zhang, Xiaojun1; Zhang, Chengsong1; Xiang, Jianhai1,3
2017-01-17
Source PublicationSCIENTIFIC REPORTS
Volume7
SubtypeArticle
AbstractWhite Spot Syndrome Virus (WSSV) is regarded as a virus with the strongest pathogenicity to shrimp. For the threshold trait such as disease resistance, marker assisted selection (MAS) was considered to be a more effective approach. In the present study, association analyses of single nucleotide polymorphisms (SNPs) located in a set of immune related genes were conducted to identify markers associated with WSSV resistance. SNPs were detected by bioinformatics analysis on RNA sequencing data generated by Illimina sequencing platform and Roche 454 sequencing technology. A total of 681 SNPs located in the exons of immune related genes were selected as candidate SNPs. Among these SNPs, 77 loci were genotyped in WSSV susceptible group and resistant group. Association analysis was performed based on logistic regression method under an additive and dominance model in GenABEL package. As a result, five SNPs showed associations with WSSV resistance at a significant level of 0.05. Besides, SNP-SNP interaction analysis was conducted. The combination of SNP loci in TRAF6, Cu/Zn SOD and nLvALF2 exhibited a significant effect on the WSSV resistance of shrimp. Gene expression analysis revealed that these SNPs might influence the expression of these immune-related genes. This study provides a useful method for performing MAS in shrimp.
DOI10.1038/srep40549
Indexed BySCI
Language英语
WOS IDWOS:000391939100001
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136289
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
First Author Affilication中国科学院海洋研究所
Recommended Citation
GB/T 7714
Yu, Yang,Liu, Jingwen,Li, Fuhua,et al. Gene set based association analyses for the WSSV resistance of Pacific white shrimp Litopenaeus vannamei[J]. SCIENTIFIC REPORTS,2017,7.
APA Yu, Yang,Liu, Jingwen,Li, Fuhua,Zhang, Xiaojun,Zhang, Chengsong,&Xiang, Jianhai.(2017).Gene set based association analyses for the WSSV resistance of Pacific white shrimp Litopenaeus vannamei.SCIENTIFIC REPORTS,7.
MLA Yu, Yang,et al."Gene set based association analyses for the WSSV resistance of Pacific white shrimp Litopenaeus vannamei".SCIENTIFIC REPORTS 7(2017).
Files in This Item:
File Name/Size DocType Version Access License
Gene set based assoc(760KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yu, Yang]'s Articles
[Liu, Jingwen]'s Articles
[Li, Fuhua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yu, Yang]'s Articles
[Liu, Jingwen]'s Articles
[Li, Fuhua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yu, Yang]'s Articles
[Liu, Jingwen]'s Articles
[Li, Fuhua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Gene set based association analyses for the WSSV resistance of Pacific white shrimp Litopenaeus vannamei.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.