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Identification and functional characterisation of 5-HT4 receptor in sea cucumber Apostichopus japonicus (Selenka)
Wang, Tianming1; Yang, Zhen1; Zhou, Naiming2; Sun, Lina3; Lv, Zhenming1; Wu, Changwen1
2017-01-06
发表期刊SCIENTIFIC REPORTS
卷号7
文章类型Article
摘要Serotonin (5-HT) is an important neurotransmitter and neuromodulator that controls a variety of sensory and motor functions through 5-HT receptors (5-HTRs). The 5-HT4R subfamily is linked to Gs proteins, which activate adenylyl cyclases (ACs), and is involved in many responses in peripheral organs. In this study, the 5-HT4R from Apostichopus japonicus (Aj5-HT4R) was identified and characterised. The cloned full-length Aj5-HT4R cDNA is 1,544 bp long and contains an open reading frame 1,011 bp in length encoding 336 amino acid proteins. Bioinformatics analysis of the Aj5-HT4R protein indicated this receptor was a member of class A G protein coupled receptor (GPCR) family. Further experiments using Aj5-HT4R-transfected HEK293 cells demonstrated that treatment with 5-HT triggered a significant increase in intracellular cAMP level in a dose-dependent manner and induced a rapid internalisation of Aj5-HT4R fused with enhanced green fluorescent protein (Aj5-HT4R-EGFP) from the cell surface into the cytoplasm. In addition, the transcriptional profiles of Aj5-HT4R in aestivating A. japonicas and phosphofructokinase (AjPFK) in 5-HT administrated A. japonicus have been analysed by real-time PCR assays. Results have led to a basic understanding of Aj5-HT4R in A. japonicus, and provide a foundation for further exploration of the cell signaling and regulatory functions of this receptor.
DOI10.1038/srep40247
收录类别SCI
语种英语
WOS记录号WOS:000391359700001
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136269
专题海洋生态与环境科学重点实验室
作者单位1.Zhejiang Ocean Univ, Marine Sci Coll, Natl Engn Res Ctr Marine Facil Aquaculture, Zhoushan 316022, Zhejiang, Peoples R China
2.Zhejiang Univ, Coll LifeSci, Inst Biochem, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
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Wang, Tianming,Yang, Zhen,Zhou, Naiming,et al. Identification and functional characterisation of 5-HT4 receptor in sea cucumber Apostichopus japonicus (Selenka)[J]. SCIENTIFIC REPORTS,2017,7.
APA Wang, Tianming,Yang, Zhen,Zhou, Naiming,Sun, Lina,Lv, Zhenming,&Wu, Changwen.(2017).Identification and functional characterisation of 5-HT4 receptor in sea cucumber Apostichopus japonicus (Selenka).SCIENTIFIC REPORTS,7.
MLA Wang, Tianming,et al."Identification and functional characterisation of 5-HT4 receptor in sea cucumber Apostichopus japonicus (Selenka)".SCIENTIFIC REPORTS 7(2017).
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