IOCAS-IR  > 实验海洋生物学重点实验室
The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin
Huang, Mengmeng1; Wang, Lingling2; Zhang, Huan3; Yang, Chuanyan2; Liu, Rui3; Xu, Jiachao3; Jia, Zhihao3; Song, Linsheng2
2017-02-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号67页码:333-339
文章类型Article
摘要A C-type lectin of multiple CRDs (CfLec-4) from Chlamys farreri was selected to investigate the sequence variation and functional differentiation of its CRDs. Its four CRDs with EPD/LSD, EPN/FAD, EPN/LND and EPN/YND key motifs were recombined separately. The recombinant proteins of CRD1 and CRD2 (designated as rCRD1 and rCRD2) could bind LPS and mannan, while the recombinant proteins of CRD3 and CRD4 (designated as rCRD3 and rCRD4) could bind LPS, PGN, mannan and glucan. Moreover, rCRD3 displayed broad microbe binding spectrum towards Gram-positive bacteria Staphylococcus aureus and Micrococcus luteus, Gram-negative bacteria Escherichia coli and Vibrio anguillarum, as well as fungi Pichia pastoris and Yarrowia lipolytica. These results indicated CRD3 contributed more to CfLec-4's nonself-recognition ability. Furthermore, CRD1, CRD3 and CRD4 functioned as opsonin participating in the clearance against invaders in scallops. The sequence variation in Ca2+ binding site 2 among CRDs was suspected to be associated with such functional differentiation. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Chlamys Farreri C-type Lectin Carbohydrate-recognition Domain Ca2++ Binding Site 2 Functional Differentiation
DOI10.1016/j.dci.2016.08.019
收录类别SCI
语种英语
WOS记录号WOS:000390071200033
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136258
专题实验海洋生物学重点实验室
作者单位1.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China
2.Dalian Ocean Univ, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Huang, Mengmeng,Wang, Lingling,Zhang, Huan,et al. The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:333-339.
APA Huang, Mengmeng.,Wang, Lingling.,Zhang, Huan.,Yang, Chuanyan.,Liu, Rui.,...&Song, Linsheng.(2017).The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,67,333-339.
MLA Huang, Mengmeng,et al."The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 67(2017):333-339.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
The sequence variati(1391KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Huang, Mengmeng]的文章
[Wang, Lingling]的文章
[Zhang, Huan]的文章
百度学术
百度学术中相似的文章
[Huang, Mengmeng]的文章
[Wang, Lingling]的文章
[Zhang, Huan]的文章
必应学术
必应学术中相似的文章
[Huang, Mengmeng]的文章
[Wang, Lingling]的文章
[Zhang, Huan]的文章
相关权益政策
暂无数据
收藏/分享
文件名: The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。