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The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin
Huang, Mengmeng1; Wang, Lingling2; Zhang, Huan3; Yang, Chuanyan2; Liu, Rui3; Xu, Jiachao3; Jia, Zhihao3; Song, Linsheng2
2017-02-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume67Pages:333-339
SubtypeArticle
AbstractA C-type lectin of multiple CRDs (CfLec-4) from Chlamys farreri was selected to investigate the sequence variation and functional differentiation of its CRDs. Its four CRDs with EPD/LSD, EPN/FAD, EPN/LND and EPN/YND key motifs were recombined separately. The recombinant proteins of CRD1 and CRD2 (designated as rCRD1 and rCRD2) could bind LPS and mannan, while the recombinant proteins of CRD3 and CRD4 (designated as rCRD3 and rCRD4) could bind LPS, PGN, mannan and glucan. Moreover, rCRD3 displayed broad microbe binding spectrum towards Gram-positive bacteria Staphylococcus aureus and Micrococcus luteus, Gram-negative bacteria Escherichia coli and Vibrio anguillarum, as well as fungi Pichia pastoris and Yarrowia lipolytica. These results indicated CRD3 contributed more to CfLec-4's nonself-recognition ability. Furthermore, CRD1, CRD3 and CRD4 functioned as opsonin participating in the clearance against invaders in scallops. The sequence variation in Ca2+ binding site 2 among CRDs was suspected to be associated with such functional differentiation. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordChlamys Farreri C-type Lectin Carbohydrate-recognition Domain Ca2++ Binding Site 2 Functional Differentiation
DOI10.1016/j.dci.2016.08.019
Indexed BySCI
Language英语
WOS IDWOS:000390071200033
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136258
Collection实验海洋生物学重点实验室
Affiliation1.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China
2.Dalian Ocean Univ, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Huang, Mengmeng,Wang, Lingling,Zhang, Huan,et al. The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:333-339.
APA Huang, Mengmeng.,Wang, Lingling.,Zhang, Huan.,Yang, Chuanyan.,Liu, Rui.,...&Song, Linsheng.(2017).The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,67,333-339.
MLA Huang, Mengmeng,et al."The sequence variation and functional differentiation of CRDs in a scallop multiple CRDs containing lectin".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 67(2017):333-339.
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