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Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas
Jiang, Shuai1; Jia, Zhihao1; Zhang, Tao1; Wang, Lingling1,2; Qiu, Limei1; Sun, Jinsheng3; Song, Linsheng2
2016-12-14
发表期刊PEERJ
卷号4
文章类型Article
摘要Invertebrates lack canonical adaptive immunity and mainly rely on innate immune system to fight against Pathogens. The phagocytes, which could engulf and kill microbial pathogens, are likely to be of great importance and have to undertake significant roles in invertebrate immune defense. In the present study, flow cytometry combined with histological and lectin staining was employed to characterise functional features of phagocytes in the Pacific oyster Crassostrea gigas. Based on the cell size and celkilar contents, haemocytes were categorised into three cell types, i.e., granulocytes, semigranulocytes and agranulocytes. Agranulocytes with smaller cell volume and lower cytoplasmic-to-nuclear ratio did not show phagocytic activity, while semigranulocytes and agranulocytes exhibited larger cell volume, higher cytoplasmic-to-nuclear ratio and phagocytic activity. In addition, granulocytes with higher side scatter (SSC) exhibited higher phagocytic activity than that of semigranulocytes. When beta-integrin and lectinlike receptors were blocked by RGD tripeptide and carbohydrates, respectively, the phagocytic activity of both granulocytes and semigranulocytes was significantly inhibited, indicating that,beta-integrin and certain lectin-like receptors were involved in phagocytosis towards microbes. Moreover, lipopolysaccharide but not peptidylglycan could enhance phagocytic activity of granulocytes and semigranulocytes towards Vibrio splendidus and Staphyloeoccus aureus. Lectin staining analysis revealed that Lyeopersicou esculentum lectin (LEL), binding the epitope polylactosaniine, was highly distributed on the extracellular cell surface of phagocytes, and could be utilized as a potential molecular marker to differentiate phagocytes from non-phagocytic haemocytes. The results, collectively, provide knowledge on the functional characters of oyster phagocytes, which would contribute to deep investigation of cell typing and cellular immunity in bivalves.
关键词Crassostrea Gigas Phagocytosis Flow Cytometry Lectin Staining Polylactosamine
DOI10.7717/peerj.2590
收录类别SCI
语种英语
WOS记录号WOS:000390054600001
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136256
专题实验海洋生物学重点实验室
海洋生态与环境科学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian, Liaoning, Peoples R China
3.Tianjin Normal Univ, Tianjin Key Lab Anim & Plant Resistance, Tianjin, Peoples R China
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GB/T 7714
Jiang, Shuai,Jia, Zhihao,Zhang, Tao,et al. Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas[J]. PEERJ,2016,4.
APA Jiang, Shuai.,Jia, Zhihao.,Zhang, Tao.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2016).Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas.PEERJ,4.
MLA Jiang, Shuai,et al."Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas".PEERJ 4(2016).
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