IOCAS-IR  > 实验海洋生物学重点实验室
Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas
Jiang, Shuai1; Jia, Zhihao1; Zhang, Tao1; Wang, Lingling1,2; Qiu, Limei1; Sun, Jinsheng3; Song, Linsheng2
2016-12-14
Source PublicationPEERJ
Volume4
SubtypeArticle
AbstractInvertebrates lack canonical adaptive immunity and mainly rely on innate immune system to fight against Pathogens. The phagocytes, which could engulf and kill microbial pathogens, are likely to be of great importance and have to undertake significant roles in invertebrate immune defense. In the present study, flow cytometry combined with histological and lectin staining was employed to characterise functional features of phagocytes in the Pacific oyster Crassostrea gigas. Based on the cell size and celkilar contents, haemocytes were categorised into three cell types, i.e., granulocytes, semigranulocytes and agranulocytes. Agranulocytes with smaller cell volume and lower cytoplasmic-to-nuclear ratio did not show phagocytic activity, while semigranulocytes and agranulocytes exhibited larger cell volume, higher cytoplasmic-to-nuclear ratio and phagocytic activity. In addition, granulocytes with higher side scatter (SSC) exhibited higher phagocytic activity than that of semigranulocytes. When beta-integrin and lectinlike receptors were blocked by RGD tripeptide and carbohydrates, respectively, the phagocytic activity of both granulocytes and semigranulocytes was significantly inhibited, indicating that,beta-integrin and certain lectin-like receptors were involved in phagocytosis towards microbes. Moreover, lipopolysaccharide but not peptidylglycan could enhance phagocytic activity of granulocytes and semigranulocytes towards Vibrio splendidus and Staphyloeoccus aureus. Lectin staining analysis revealed that Lyeopersicou esculentum lectin (LEL), binding the epitope polylactosaniine, was highly distributed on the extracellular cell surface of phagocytes, and could be utilized as a potential molecular marker to differentiate phagocytes from non-phagocytic haemocytes. The results, collectively, provide knowledge on the functional characters of oyster phagocytes, which would contribute to deep investigation of cell typing and cellular immunity in bivalves.
KeywordCrassostrea Gigas Phagocytosis Flow Cytometry Lectin Staining Polylactosamine
DOI10.7717/peerj.2590
Indexed BySCI
Language英语
WOS IDWOS:000390054600001
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136256
Collection实验海洋生物学重点实验室
海洋生态与环境科学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian, Liaoning, Peoples R China
3.Tianjin Normal Univ, Tianjin Key Lab Anim & Plant Resistance, Tianjin, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jiang, Shuai,Jia, Zhihao,Zhang, Tao,et al. Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas[J]. PEERJ,2016,4.
APA Jiang, Shuai.,Jia, Zhihao.,Zhang, Tao.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2016).Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas.PEERJ,4.
MLA Jiang, Shuai,et al."Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas".PEERJ 4(2016).
Files in This Item:
File Name/Size DocType Version Access License
Functional character(6605KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jiang, Shuai]'s Articles
[Jia, Zhihao]'s Articles
[Zhang, Tao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jiang, Shuai]'s Articles
[Jia, Zhihao]'s Articles
[Zhang, Tao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jiang, Shuai]'s Articles
[Jia, Zhihao]'s Articles
[Zhang, Tao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Functional characterisation of phagocytes in the Pacific oyster Crassostrea gigas.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.