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The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas
Wang, Weilin1,3; Li, Meijia1,3; Wang, Lingling2; Chen, Hao1,3; Liu, Zhaoqun1,3; Jia, Zhihao1,3; Qiu, Limei1; Song, Linsheng1,2
AbstractHemocytes comprise diverse cell types with morphological and functional heterogeneity and play indispensable roles in immunological homeostasis of invertebrates. The morphological classification of different hemocytes in mollusk has been studied since the 1970's, yet the involvement of the different sub-populations in immune functions is far from clear. In the present study, three types of hemocytes were morphologically identified and separated as agranulocytes, semi-granulocytes and granulocytes by flow cytometry and Percoll density gradient centrifugation. The granulocytes were characterized functionally as the main phagocytic and encapsulating population, while semi-granulocytes and agranulocytes exhibited low or no such capacities, respectively. Meanwhile, the lysosome activity and the productions of ROS and NO were all mainly concentrated in granulocytes under both normal and immune-activated situations. Further, the mRNA transcripts of some immune related genes, including CgTLR, CgClathrin, CgATPeV, CgLysozyme, CgDefensin and CgIL-17, were mainly expressed in granulocytes, lower in semi-granulocytes and agranulocytes. These results collectively suggested that the granulocytes were the main immunocompetent hemocytes in oyster C. gigas, and a differentiation relationship among these three sub-population hemocytes was inferred based on the gradual changes in morphological, functional and molecular features. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Hemocyte Separation Granulocytes Immunocompetent Functional Features
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WOS IDWOS:000390071200021
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Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
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Wang, Weilin,Li, Meijia,Wang, Lingling,et al. The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:221-228.
APA Wang, Weilin.,Li, Meijia.,Wang, Lingling.,Chen, Hao.,Liu, Zhaoqun.,...&Song, Linsheng.(2017).The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,67,221-228.
MLA Wang, Weilin,et al."The granulocytes are the main immunocompetent hemocytes in Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 67(2017):221-228.
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