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Effect of smokeless tobacco products on human oral bacteria growth and viability
Liu, Min1,2; Jin, Jinshan1; Pan, Hongmiao1,3; Feng, Jinhui1,4; Cerniglia, Carl E.1; Yang, Maocheng5; Chen, Huizhong1
2016-12-01
Source PublicationANAEROBE
Volume42Pages:152-161
SubtypeArticle
AbstractTo evaluate the toxicity of smokeless tobacco products (STPs) on oral bacteria, seven smokeless tobacco aqueous extracts (STAEs) from major brands of STPs and three tobacco-specific N-nitrosamines (TSNAs) were used in a growth and viability test against 38 oral bacterial species or subspecies. All seven STAEs showed concentration-dependent effects on the growth and viability of tested oral bacteria under anaerobic culture conditions, although there were strain-to-strain variations. In the presence of 1 mg/ml STAEs, the growth of 4 strains decreased over 0.32-2.14 log(10) fold, while 14 strains demonstrated enhanced growth of 0.3-1.76 log(10) fold, and the growth of 21 strains was not significantly affected. In the presence of 10 mg/ml STAE5, the growth of 17 strains was inhibited 0.3-2.11 log(10) fold, 18 strains showed enhanced growth of 0.3-0.97 log(10) fold, and 4 strains were not significantly affected. In the presence of 50 mg/ml STAEs, the growth of 32 strains was inhibited 0.3-2.96 log(10) fold, 8 strains showed enhanced growth of 0.3-1.0 log(10) fold, and 2 strains were not significantly affected. All seven STAEs could promote the growth of 4 bacterial strains, including Eubacterium nodatum, Peptostreptococcus micros, Streptococcus anginosus, and Streptococcus constellatus. Exposure to STAEs modulated the viability of some bacterial strains, with 21.1-66.5% decrease for 4 strains at 1 mg/ml, 20.3-85.7% decrease for 10 strains at 10 mg/ml, 20.0-93.3% decrease for 27 strains at 50 mg/ml, and no significant effect for 11 strains at up to 50 mg/ml. STAE5 from snuffs inhibited more tested bacterial strains than those from snus indicating that the snuffs may be more toxic to the oral bacteria than snus. For TSNAs, cell growth and viability of 34 tested strains were not significantly affected at up to 100 mu g/ml; while the growth of P. micros was enhanced 0.31-0.54 log(10) fold; the growth of Veillonella parvula was repressed 0.33-0.36 log(10) fold; and the cell viabilities of 2 strains decreased 56.6-69.9%. The results demonstrate that STAE5 affected the growth of some types of oral bacteria, which may affect the healthy ecological balance of oral bacteria in humans. On the other hand, TSNAs did not significantly affect the growth of the oral bacteria. Published by Elsevier Ltd.
KeywordSmokeless Tobacco Toxicology Oral Bacteria Cell Viability Tobacco-specific N-nitrosamines
DOI10.1016/j.anaerobe.2016.10.006
Indexed BySCI
Language英语
WOS IDWOS:000390628600027
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136248
Collection海洋生态与环境科学重点实验室
Affiliation1.USDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
5.USDA, Off Sci, Ctr Tobacco Prod, 10903 New Hampshire Ave, Silver Spring, MD 20993 USA
Recommended Citation
GB/T 7714
Liu, Min,Jin, Jinshan,Pan, Hongmiao,et al. Effect of smokeless tobacco products on human oral bacteria growth and viability[J]. ANAEROBE,2016,42:152-161.
APA Liu, Min.,Jin, Jinshan.,Pan, Hongmiao.,Feng, Jinhui.,Cerniglia, Carl E..,...&Chen, Huizhong.(2016).Effect of smokeless tobacco products on human oral bacteria growth and viability.ANAEROBE,42,152-161.
MLA Liu, Min,et al."Effect of smokeless tobacco products on human oral bacteria growth and viability".ANAEROBE 42(2016):152-161.
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