Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
CsMAP34, a teleost MAP with dual role: A promoter of MASP-assisted complement activation and a regulator of immune cell activity | |
Li, Mo-fei1,2; Li, Jun3; Sun, Li1,2 | |
2016-12-23 | |
发表期刊 | SCIENTIFIC REPORTS |
卷号 | 6 |
文章类型 | Article |
摘要 | In teleost fish, the immune functions of mannan-binding lectin (MBL) associated protein (MAP) and MBL associated serine protease (MASP) are scarcely investigated. In the present study, we examined the biological properties both MAP (CsMAP34) and MASP (CsMASP1) molecules from tongue sole (Cynoglossus semilaevis). We found that CsMAP34 and CsMASP1 expressions occurred in nine different tissues and were upregulated by bacterial challenge. CsMAP34 protein was detected in blood, especially during bacterial infection. Recombinant CsMAP34 (rCsMAP34) bound C. semilaevis MBL (rCsBML) when the latter was activated by bacteria, while recombinant CsMASP1 (rCsMASP1) bound activated rCsBML only in the presence of rCsMAP34. rCsMAP34 stimulated the hemolytic and bactericidal activities of serum complement, whereas anti-CsMAP34 antibody blocked complement activities. Knockdown of CsMASP1 in C. semilaevis resulted in significant inhibition of complement activities. Furthermore, rCsMAP34 interacted directly with peripheral blood leukocytes (PBL) and enhanced the respiratory burst, acid phosphatase activity, chemotactic activity, and gene expression of PBL. These results indicate for the first time that a teleost MAP acts one hand as a regulator that promotes the lectin pathway of complement activation via its ability to recruit MBL to MASP, and other hand as a modulator of immune cell activity. |
DOI | 10.1038/srep39287 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000390373900001 |
引用统计 | |
文献类型 | 期刊论文 |
版本 | 出版稿 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/136246 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI USA |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Li, Mo-fei,Li, Jun,Sun, Li. CsMAP34, a teleost MAP with dual role: A promoter of MASP-assisted complement activation and a regulator of immune cell activity[J]. SCIENTIFIC REPORTS,2016,6. |
APA | Li, Mo-fei,Li, Jun,&Sun, Li.(2016).CsMAP34, a teleost MAP with dual role: A promoter of MASP-assisted complement activation and a regulator of immune cell activity.SCIENTIFIC REPORTS,6. |
MLA | Li, Mo-fei,et al."CsMAP34, a teleost MAP with dual role: A promoter of MASP-assisted complement activation and a regulator of immune cell activity".SCIENTIFIC REPORTS 6(2016). |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
CsMAP34, a teleost M(2101KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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