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Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas
Jia, Zhihao1,3; Zhang, Huan1; Jiang, Shuai1; Wang, Mengqiang1; Wang, Lingling2; Song, Linsheng2
2016-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume59Pages:220-232
SubtypeArticle
AbstractC-type lectins (CTLs), a superfamily of Cat(2+)-dependent carbohydrate-recognition proteins, are involved in nonself-recognition and pathogen elimination, and play crucial roles in the innate immunity. In the present study, two single CRD C-type lectins, CgCLec-4 and CgCLec-5, were identified from oyster Crassostrea gigas. The open reading frame (ORF) of CgCLec-4 and CgCLec-5 encoded polypeptides of 152 and 150 amino acids, respectively. Both CgCLec-4 and CgCLec-5 contained one CRD with six conserved cysteines to form three disulfide bridges. The motif in Ca2+-binding site 2 of CgCLec-4 was QPE, while it was QYE, a non-a typical motif in CgCLec-5. CgCLec-4 was a secreted lectin with a signal peptide which was highly expressed in hepatopancreas, mantle and hemocytes. CgCLec-5 was an intracellular lectin which was mostly expressed in hemocytes. The lipopolysaccharide stimulation could induce the expressions of CgCLec-4 and CgCLec-5. The recombinant proteins of CgCLec-4 and CgCLec-5 (rCgCLec-4 and rCgCLec-5) could bind to various PAMPs including LPS, PGN, GLU and mannan, while the binding affinity of rCgCLec-5 was stronger than that of rCgCLec-4. Meanwhile, rCgCLec-4 and rCgCLec-5 could bind to different kinds of microorganisms, including Staphylococcus aureus, Escherichia coli and Vibro anguillarum and Yarrowia lipolytica, and the microbial agglutinating ability of rCgCLec-4 was stronger than that of CgCLec-5. Moreover, rCgCLec-4 exhibited anti-microbial activity against bacteria and fungi, but antimicrobial activity of CgCLec-5 was not obvious. All these results suggested that CgCLec-4 and CgCLec-5 could function as an important PRR involved in immune defense against invading pathogen in oyster, and the diversity and complexity of motifs in Ca2+ binding site 2 in CRDs determined their comprehensive recognition spectrum and multiple immune functions. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Innate Immunity Lectin Recognition Antimicrobial Activity
DOI10.1016/j.fsi.2016.10.030
Indexed BySCI
Language英语
WOS IDWOS:000389729100025
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136238
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Zhihao,Zhang, Huan,Jiang, Shuai,et al. Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,59:220-232.
APA Jia, Zhihao,Zhang, Huan,Jiang, Shuai,Wang, Mengqiang,Wang, Lingling,&Song, Linsheng.(2016).Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,59,220-232.
MLA Jia, Zhihao,et al."Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 59(2016):220-232.
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