Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas

doi:10.1016/j.fsi.2016.10.030

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	Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas
	Jia, Zhihao 1,3; Zhang, Huan 1; Jiang, Shuai 1; Wang, Mengqiang1 ; Wang, Lingling 2; Song, Linsheng 2
	2016-12-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	59 页码:220-232
文章类型	Article
摘要	C-type lectins (CTLs), a superfamily of Cat(2+)-dependent carbohydrate-recognition proteins, are involved in nonself-recognition and pathogen elimination, and play crucial roles in the innate immunity. In the present study, two single CRD C-type lectins, CgCLec-4 and CgCLec-5, were identified from oyster Crassostrea gigas. The open reading frame (ORF) of CgCLec-4 and CgCLec-5 encoded polypeptides of 152 and 150 amino acids, respectively. Both CgCLec-4 and CgCLec-5 contained one CRD with six conserved cysteines to form three disulfide bridges. The motif in Ca2+-binding site 2 of CgCLec-4 was QPE, while it was QYE, a non-a typical motif in CgCLec-5. CgCLec-4 was a secreted lectin with a signal peptide which was highly expressed in hepatopancreas, mantle and hemocytes. CgCLec-5 was an intracellular lectin which was mostly expressed in hemocytes. The lipopolysaccharide stimulation could induce the expressions of CgCLec-4 and CgCLec-5. The recombinant proteins of CgCLec-4 and CgCLec-5 (rCgCLec-4 and rCgCLec-5) could bind to various PAMPs including LPS, PGN, GLU and mannan, while the binding affinity of rCgCLec-5 was stronger than that of rCgCLec-4. Meanwhile, rCgCLec-4 and rCgCLec-5 could bind to different kinds of microorganisms, including Staphylococcus aureus, Escherichia coli and Vibro anguillarum and Yarrowia lipolytica, and the microbial agglutinating ability of rCgCLec-4 was stronger than that of CgCLec-5. Moreover, rCgCLec-4 exhibited anti-microbial activity against bacteria and fungi, but antimicrobial activity of CgCLec-5 was not obvious. All these results suggested that CgCLec-4 and CgCLec-5 could function as an important PRR involved in immune defense against invading pathogen in oyster, and the diversity and complexity of motifs in Ca2+ binding site 2 in CRDs determined their comprehensive recognition spectrum and multiple immune functions. (C) 2016 Elsevier Ltd. All rights reserved.
关键词	Crassostrea Gigas Innate Immunity Lectin Recognition Antimicrobial Activity
DOI	10.1016/j.fsi.2016.10.030
收录类别	SCI
语种	英语
WOS记录号	WOS:000389729100025
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136238
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Jia, Zhihao,Zhang, Huan,Jiang, Shuai,et al. Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,59:220-232.
APA	Jia, Zhihao,Zhang, Huan,Jiang, Shuai,Wang, Mengqiang,Wang, Lingling,&Song, Linsheng.(2016).Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,59,220-232.
MLA	Jia, Zhihao,et al."Comparative study of two single CRD C-type lectins, CgCLec-4 and CgCLec-5, from pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 59(2016):220-232.

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