Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase

doi:10.1016/j.ijbiomac.2016.08.031

IOCAS-IR > 实验海洋生物学重点实验室

	Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase
	Wang, Xueyan 1; Duan, Delin 2; Fu, Xiaoting 1
	2016-12-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷号	93 页码:600-608
文章类型	Article
摘要	Agar and sulfated galactans were isolated from the red seaweeds Gracilariopsis lemaneiformis and Gelidium amansii. A previously purified arylsulfatase from Marinomonas sp. FW-1 was used to remove sulfate groups in agar and sulfated galactans. After enzymatic desulfation, the sulfate content decreased to about 0.16% and gel strength increased about two folds. Moreover, there was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated agar and that of the commercial agarose. In order to reveal the desulfation ratio and site, chemical and structural identification of sulfated galactan were carried out. G. amansii sulfated galactan with 7.4% sulfated content was composed of galactose and 3,6-anhydro-L-galactose. Meanwhile, G. lemaneiformis sulfated galactan with 8.5% sulfated content was composed of galactose, 3,6-anhydro-L-galactose, 2-O-methyl-3,6-anhydro-L-galactose and xylose. Data from C-13 NMR, FT-IR, GC-MS provided evidence of sulfate groups at C-4 and C-6 of d-galactose and C-6 of I-galactose both in GRAP and GEAP. Data from GC-MS revealed that desulfation was carried out by the arylsulfatase at the sulfate bonds at C-4 and C-6 of d-galactose and C-6 of l-galactose, with a desulfation ratio of 83.4% and 86.0% against GEAP and GRAP, respectively. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Arylsulfatase Agarose Desulfation Sulfated Galactan Spectroscopy
DOI	10.1016/j.ijbiomac.2016.08.031
收录类别	SCI
语种	英语
WOS记录号	WOS:000389090900070
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136232
专题	实验海洋生物学重点实验室
作者单位	1.Ocean Univ China, Coll Food Sci & Engn, 5 Yushan Rd, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xueyan,Duan, Delin,Fu, Xiaoting. Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,93:600-608.
APA	Wang, Xueyan,Duan, Delin,&Fu, Xiaoting.(2016).Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,93,600-608.
MLA	Wang, Xueyan,et al."Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 93(2016):600-608.

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