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Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase
Wang, Xueyan1; Duan, Delin2; Fu, Xiaoting1
2016-12-01
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷号93页码:600-608
文章类型Article
摘要Agar and sulfated galactans were isolated from the red seaweeds Gracilariopsis lemaneiformis and Gelidium amansii. A previously purified arylsulfatase from Marinomonas sp. FW-1 was used to remove sulfate groups in agar and sulfated galactans. After enzymatic desulfation, the sulfate content decreased to about 0.16% and gel strength increased about two folds. Moreover, there was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated agar and that of the commercial agarose. In order to reveal the desulfation ratio and site, chemical and structural identification of sulfated galactan were carried out. G. amansii sulfated galactan with 7.4% sulfated content was composed of galactose and 3,6-anhydro-L-galactose. Meanwhile, G. lemaneiformis sulfated galactan with 8.5% sulfated content was composed of galactose, 3,6-anhydro-L-galactose, 2-O-methyl-3,6-anhydro-L-galactose and xylose. Data from C-13 NMR, FT-IR, GC-MS provided evidence of sulfate groups at C-4 and C-6 of d-galactose and C-6 of I-galactose both in GRAP and GEAP. Data from GC-MS revealed that desulfation was carried out by the arylsulfatase at the sulfate bonds at C-4 and C-6 of d-galactose and C-6 of l-galactose, with a desulfation ratio of 83.4% and 86.0% against GEAP and GRAP, respectively. (C) 2016 Elsevier B.V. All rights reserved.
关键词Arylsulfatase Agarose Desulfation Sulfated Galactan Spectroscopy
DOI10.1016/j.ijbiomac.2016.08.031
收录类别SCI
语种英语
WOS记录号WOS:000389090900070
引用统计
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136232
专题实验海洋生物学重点实验室
作者单位1.Ocean Univ China, Coll Food Sci & Engn, 5 Yushan Rd, Qingdao 266003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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Wang, Xueyan,Duan, Delin,Fu, Xiaoting. Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,93:600-608.
APA Wang, Xueyan,Duan, Delin,&Fu, Xiaoting.(2016).Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,93,600-608.
MLA Wang, Xueyan,et al."Enzymatic desulfation of the red seaweeds agar by Marinomonas arylsulfatase".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 93(2016):600-608.
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