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Polymorphism in a serine protease inhibitor gene and its association with the resistance of bay scallop (Argopecten irradians) to Listonella anguillarum challenge
Siva, Vinu S.1,2,3; Wang, Lingling1; Qiu, Limei2; Zhou, Zhi2; Liu, Chao1; Yang, Jialong2; Yang, Chuanyan1; Song, Linsheng1
2016-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume59Pages:1-8
SubtypeArticle
AbstractSerine protease inhibitors (SPIs) play a crucial role in regulation of both host and bacterial serine protease. They are classified into several protein families, where Kazal-type inhibitors are one of families with multi-domain. In the present study, the polymorphism of AiSPI from Bay scallop Argopecten irradians was found to be associated with disease resistance of bay scallop against Listonella anguillarum. Nine single nucleotide polymorphisms (SNPs) were identified in the exon region of AiSPI, where five SNPs were non-synonymous mutation. Three of these mutations were located in "kazal-like 3"domain, two SNP loci positioned at +536, +1312 were selected for further association studies. For the locus +536, the genotype frequency of A/G in the resistant stock (12.8%) was significantly lower (p < 0.05) than that in the susceptible stock (35.1%), while, the genotype A/A in the resistant stock (87.2%) was significantly higher in comparison with susceptible stock (64.9%) (p < 0.05). The G allele frequencies were 6.4% and 17.6% in resistant stock and susceptible stock, respectively, and chi(2)-test revealed a significant difference in the frequency distribution between the two stocks (p < 0.05). But there was no significant association between the mutation C-T at locus +1312 with either resistant or susceptible group (p > 0.05). The genotype frequencies of T/T, T/C, C/C at locus +1312 were 94.6%, 2.7% and 2.7% respectively in the susceptible stock, while 100%, 0% and 0% respectively in the resistant stock. The amino acid change for the mutation at locus +536 A-G was from asparagine to serine, and the predicted homology model of this amino acid variation could affect its function as well as the structural integrity of the domain. In vitro elastase inhibition assay of the protein variants at locus +536 was conducted to explicate the effect of SNP. The increasing concentration of protein (0 mmol/L- 2.93 mmol/L) was incubated with 80 nmol/L elastase where the residual enzyme activity values for rAiSPI (N) with A variant and rAiSPI (S) with G variant were started to reduce from 0.40 to 0.215 and 0.435 to 0.356, respectively. The elastase inhibition ability of rAiSPI (N) variant was significantly higher than that of rAiSPI (S) (p < 0.01). The results suggested that the mutation at locus +536A/A significantly associated with disease resistance of bay scallop would shed light for selective breeding program. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordArgopecten Irradians Bay Scallop Single Nucleotide Polymorphism Serine Protease Inhibitor Disease Resistance Disease Susceptibility
DOI10.1016/j.fsi.2016.09.056
Indexed BySCI
Language英语
WOS IDWOS:000389729100001
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136231
Collection实验海洋生物学重点实验室
Affiliation1.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Sathyabama Univ, Ctr Climate Change Studies, Madras 600119, Tamil Nadu, India
Recommended Citation
GB/T 7714
Siva, Vinu S.,Wang, Lingling,Qiu, Limei,et al. Polymorphism in a serine protease inhibitor gene and its association with the resistance of bay scallop (Argopecten irradians) to Listonella anguillarum challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2016,59:1-8.
APA Siva, Vinu S..,Wang, Lingling.,Qiu, Limei.,Zhou, Zhi.,Liu, Chao.,...&Song, Linsheng.(2016).Polymorphism in a serine protease inhibitor gene and its association with the resistance of bay scallop (Argopecten irradians) to Listonella anguillarum challenge.FISH & SHELLFISH IMMUNOLOGY,59,1-8.
MLA Siva, Vinu S.,et al."Polymorphism in a serine protease inhibitor gene and its association with the resistance of bay scallop (Argopecten irradians) to Listonella anguillarum challenge".FISH & SHELLFISH IMMUNOLOGY 59(2016):1-8.
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