CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda

doi:10.1534/g3.116.034082

IOCAS-IR > 实验海洋生物学重点实验室

	CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda
	Gui, Tianshu1,2 ; Zhang, Jiquan 1,3; Song, Fengge1,2 ; Sun, Yuying 1,4; Xie, Shijun1,2 ; Yu, Kuijie 1; Xiang, Jianhai1,3
	2016-11-01
发表期刊	G3-GENES GENOMES GENETICS
卷号	6 期号:11 页码:3757-3764
文章类型	Article
摘要	The development of the type II clustered regularly interspaced short palindromic repeats (CRISPR) system has resulted in the revolution of genetic engineering, and this technology has been applied in the genome editing of various species. However, there are no reports about target-specific genome editing in shrimp. In this research, we developed a microinjection method for the ridgetail white prawn Exopalaemon carinicauda and successfully applied CRISPR/Cas9 technology to the genome editing of E. carinicauda. Through coinjection of mRNA of Cas9 nuclease and gRNA specialized for E. carinicauda chitinase 4 (EcChi4), shrimps with indel mutations were obtained. Further analysis showed that the mutations could be transmitted to the next generation. This is the first time that site-specific genome editing has been successfully demonstrated in a decapod, and will further contribute to the study of functional genomics in decapods.
关键词	Microinjection Exopalaemon Carinicauda Crispr/cas9 Chitinase
DOI	10.1534/g3.116.034082
收录类别	SCI
语种	英语
WOS记录号	WOS:000388678000032
引用统计	被引频次：53[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136228
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 4.Huaihai Inst Technol, Coll Marine Life & Fisheries, Lianyungang 222005, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Gui, Tianshu,Zhang, Jiquan,Song, Fengge,et al. CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda[J]. G3-GENES GENOMES GENETICS,2016,6(11):3757-3764.
APA	Gui, Tianshu.,Zhang, Jiquan.,Song, Fengge.,Sun, Yuying.,Xie, Shijun.,...&Xiang, Jianhai.(2016).CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda.G3-GENES GENOMES GENETICS,6(11),3757-3764.
MLA	Gui, Tianshu,et al."CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda".G3-GENES GENOMES GENETICS 6.11(2016):3757-3764.

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