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CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda
Gui, Tianshu1,2; Zhang, Jiquan1,3; Song, Fengge1,2; Sun, Yuying1,4; Xie, Shijun1,2; Yu, Kuijie1; Xiang, Jianhai1,3
2016-11-01
发表期刊G3-GENES GENOMES GENETICS
卷号6期号:11页码:3757-3764
文章类型Article
摘要The development of the type II clustered regularly interspaced short palindromic repeats (CRISPR) system has resulted in the revolution of genetic engineering, and this technology has been applied in the genome editing of various species. However, there are no reports about target-specific genome editing in shrimp. In this research, we developed a microinjection method for the ridgetail white prawn Exopalaemon carinicauda and successfully applied CRISPR/Cas9 technology to the genome editing of E. carinicauda. Through coinjection of mRNA of Cas9 nuclease and gRNA specialized for E. carinicauda chitinase 4 (EcChi4), shrimps with indel mutations were obtained. Further analysis showed that the mutations could be transmitted to the next generation. This is the first time that site-specific genome editing has been successfully demonstrated in a decapod, and will further contribute to the study of functional genomics in decapods.
关键词Microinjection Exopalaemon Carinicauda Crispr/cas9 Chitinase
DOI10.1534/g3.116.034082
收录类别SCI
语种英语
WOS记录号WOS:000388678000032
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文献类型期刊论文
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条目标识符http://ir.qdio.ac.cn/handle/337002/136228
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
4.Huaihai Inst Technol, Coll Marine Life & Fisheries, Lianyungang 222005, Peoples R China
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Gui, Tianshu,Zhang, Jiquan,Song, Fengge,et al. CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda[J]. G3-GENES GENOMES GENETICS,2016,6(11):3757-3764.
APA Gui, Tianshu.,Zhang, Jiquan.,Song, Fengge.,Sun, Yuying.,Xie, Shijun.,...&Xiang, Jianhai.(2016).CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda.G3-GENES GENOMES GENETICS,6(11),3757-3764.
MLA Gui, Tianshu,et al."CRISPR/Cas9-Mediated Genome Editing and Mutagenesis of EcChi4 in Exopalaemon carinicauda".G3-GENES GENOMES GENETICS 6.11(2016):3757-3764.
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