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Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix
Wang, Xiudan1,2,3; Lin, Hong1; Cao, Limin1; Zheng, Hongwei1; Cui, Mengqi1; Du, Shuyuan1; Sui, Jianxin1
2016-10-01
Source PublicationFOOD SCIENCE AND BIOTECHNOLOGY
Volume25Issue:5Pages:1265-1273
SubtypeArticle
AbstractThe interaction between some proteins and immune globulin has been confirmed as an important source of matrix interference with the immunoassay of fishery products, but detailed biochemical properties of these proteins have not been indicated. Two interference-inducing proteins (42 and 36 kD) in flounder were isolated, characterized, and identified. Their influences on the immunoassay of norfloxacin were confirmed by western blotting and enzyme-linked immunosorbent assay. The pI value and pH stability of the two proteins were also investigated. Using LC-MS/MS, the two proteins were identified as fructose-bisphosphate aldolase, and such results were partly verified by the aldolase activity of the 42 and 36 kD isolates. Considering the prevalence of these proteins (as multi-functional aldolase of muscles) in foods, these results would help to further understand the matrix effects in various immunoassays as well as the development of effective techniques to improve the efficiency of immunoassays.
KeywordMatrix Interference Fish Protein Enzyme-linked Immunosorbent Assay Biochemical Property Aldolase
DOI10.1007/s10068-016-0200-x
Indexed BySCI
Language英语
WOS IDWOS:000387529400006
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136210
Collection实验海洋生物学重点实验室
Affiliation1.Ocean Univ China, Food Safety Lab, Qingdao 266003, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xiudan,Lin, Hong,Cao, Limin,et al. Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix[J]. FOOD SCIENCE AND BIOTECHNOLOGY,2016,25(5):1265-1273.
APA Wang, Xiudan.,Lin, Hong.,Cao, Limin.,Zheng, Hongwei.,Cui, Mengqi.,...&Sui, Jianxin.(2016).Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix.FOOD SCIENCE AND BIOTECHNOLOGY,25(5),1265-1273.
MLA Wang, Xiudan,et al."Isolation, characterization, and identification of proteins interfering with enzyme-linked immunosorbent assay of antibiotics in fish matrix".FOOD SCIENCE AND BIOTECHNOLOGY 25.5(2016):1265-1273.
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