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CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response
Liu, Zhaoqun1,3; Zhou, Zhi1; Wang, Lingling2; Qiu, Limei1; Zhang, Huan1; Wang, Hao1; Song, Linsheng2
2016-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume58Pages:50-58
SubtypeArticle
AbstractWe have now cloned an alpha-1 adrenergic receptor (A1AR) from the cDNA library of oyster Crassostrea gigas, designating as CgA1AR-1. The full length of CgA1AR-1 was 1149 bp and it encodes a protein of 382 amino acids containing a 7 transmembrane domain, whose putative topology was similar to the A1ARs in higher organisms and shared similarity of 19% with mammalian A1ARs according to the phylogenic analysis. After cell transfection of CgA1AR-1 into HEK293T cells and the incubation with its specific agonist norepinephrine (NE), the concentration of second messenger Ca2+ increased significantly (p < 0.05). But, this increasing of Ca2+ could be inhibited by adding AJAR antagonist DOX. Tissue distribution assays using qRT-PCR suggested that CgA1AR-1 mRNA was ubiquitously expressed in all the major tissues of oyster. LPS stimulation could induce the up-regulation of CgA1AR-1 mRNA in haemocytes from 12 h to 24 h post stimulation. Moreover, the blocking of CgA1AR-1 by DOX before LPS stimulation affected the mRNA expression of oyster TNF (CGI_10005109 and CGL_10006440) in haemocytes, resulting in the rise of haemocyte phagocytic rate and apoptosis index. In addition to cellular immunity, CgA1AR-1 was also involved in humoral immunity of oyster. Inhibition of CgA1AR-1 with DOX could repress the up-regulation of LZY and SOD activities caused by LPS stimulation. These results suggested that CgA1AR-1 acted as an alpha-1 adrenergic receptor in cetacholaminergic neuroendocrineimmune network mediating both cellular and humoral immune response. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCrassostrea Gigas Norepinephrine Alpha-1 Adrenergic Receptor Tnf Pathway Immune Regulation
DOI10.1016/j.fsi.2016.09.022
Indexed BySCI
Language英语
WOS IDWOS:000387523100007
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136200
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Zhaoqun,Zhou, Zhi,Wang, Lingling,et al. CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:50-58.
APA Liu, Zhaoqun.,Zhou, Zhi.,Wang, Lingling.,Qiu, Limei.,Zhang, Huan.,...&Song, Linsheng.(2016).CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response.FISH & SHELLFISH IMMUNOLOGY,58,50-58.
MLA Liu, Zhaoqun,et al."CgA1AR-1 acts as an alpha-1 adrenergic receptor in oyster Crassostrea gigas mediating both cellular and humoral immune response".FISH & SHELLFISH IMMUNOLOGY 58(2016):50-58.
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