CD94 of tongue sole Cynoglossus semilaevis binds a wide arrange of bacteria and possesses antibacterial activity

doi:10.1016/j.fsi.2016.10.005

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	CD94 of tongue sole Cynoglossus semilaevis binds a wide arrange of bacteria and possesses antibacterial activity
	Li, Xue-peng1,2,3 ; Hu, Yong-hua 1,2
	2016-11-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	58 页码:641-649
文章类型	Article
摘要	In this study, we examined the expression patterns and the functions of the tongue sole Cynoglossus semilaevis CD94, CsCD94. CsCD94 is composed of 209 amino acid residues and shares 43.0-50.2% overall identities with known teleost CD94 sequence. CsCD94 has a C-type lectin-like domain. Expression of CsCD94 occurred in multiple tissues and was upregulated during bacterial infection. Recombinant CsCD94 (rCsCD94) exhibited apparent binding and agglutinating activities against both Gram-positive and Gram-negative bacteria in a Ca2+-dependent manner. Treatment of bacteria with rCsCD94 enhanced phagocytosis of the bacteria by peripheral blood leukocytes. Furthermore, incubation of rCsCD94 with bacteria reduced the survival of the bacteria in vitro. Taken together, these results indicate that rCsCD94 is a key factor in the bactericidal and phagocytic effects of tongue sole, and reveal for the first time an essential role of fish CD94 in antibacterial immunity, thereby adding insight into the function of CD94. (C) 2016 Elsevier Ltd. All rights reserved.
关键词	Cd94 Cynoglossus Semilaevis Agglutination Phagocytosis Antibacterial
DOI	10.1016/j.fsi.2016.10.005
收录类别	SCI
语种	英语
WOS记录号	WOS:000387523100074
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136199
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Xue-peng,Hu, Yong-hua. CD94 of tongue sole Cynoglossus semilaevis binds a wide arrange of bacteria and possesses antibacterial activity[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:641-649.
APA	Li, Xue-peng,&Hu, Yong-hua.(2016).CD94 of tongue sole Cynoglossus semilaevis binds a wide arrange of bacteria and possesses antibacterial activity.FISH & SHELLFISH IMMUNOLOGY,58,641-649.
MLA	Li, Xue-peng,et al."CD94 of tongue sole Cynoglossus semilaevis binds a wide arrange of bacteria and possesses antibacterial activity".FISH & SHELLFISH IMMUNOLOGY 58(2016):641-649.

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