IOCAS-IR  > 海洋生物技术研发中心
Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR
Liu, Peng-fei1,2; Du, Yishuai1; Meng, Lingjie1,2; Li, Xian1; Liu, Ying1,3
2016-11-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号58页码:292-301
文章类型Article
摘要Aeromonas salmonicida, an important pathogenic bacterium which induces furunculosis, is globally causing increased risks in Atlantic salmon (Salmo salar) farming. Although the kidney is the main target organ of A. salmonicida, the metabolic profiling of kidney in response to A. salmonicida in vivo remains unknown. Here, we used H-1 nuclear magnetic resonance (NMR) to comprehensively analyze the metabolic changes in the kidney of Atlantic salmon. Through the NOESYPRID spectrum combined with multi-variate pattern recognition analysis, including principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) models, significant metabolic changes were observed seven and 14 days post-infection and in a control group. Hence, the main objective of this study was to estimate the significant metabolites with resistance to furunculosis and further understand the mechanism of A. salmonicida in Atlantic salmon. Notably, substantial alterations of kidney metabolites were observed, such as with fumarate, alanine, valine, glycine, aspartate, choline, glycerophosphocholine and betaine; and summarized by metabolic pathways including the citrate cycle, glycolysis/gluconeo-genesis, tryptophan metabolism, and urea cycle, respectively. Changes were also observed in 3-hydroxybutyrate and phosphocholine which were not involved in these four metabolic pathways. After analyzing the alteration trend of these metabolites, we inferred that A. salmonicida caused absorption inhibition of amino acids and disturbed protein metabolism as well as cell metabolism in favor of its replication. These observations offered novel insights into the mechanisms of infection at a functional level and facilitated further assessment and clarification of fish disease from A. salmonicida exposure. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Metabolomics Nmr H-1 Nmr Spectroscopy Aeromonas Salmonicida Atlantic Salmon
DOI10.1016/j.fsi.2016.08.055
收录类别SCI
语种英语
WOS记录号WOS:000387523100035
引用统计
被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136193
专题海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Dalian Ocean Univ, Dalian, Peoples R China
第一作者单位中国科学院海洋研究所
推荐引用方式
GB/T 7714
Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR[J]. FISH & SHELLFISH IMMUNOLOGY,2016,58:292-301.
APA Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,Li, Xian,&Liu, Ying.(2016).Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR.FISH & SHELLFISH IMMUNOLOGY,58,292-301.
MLA Liu, Peng-fei,et al."Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR".FISH & SHELLFISH IMMUNOLOGY 58(2016):292-301.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Metabolic profiling (2473KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Peng-fei]的文章
[Du, Yishuai]的文章
[Meng, Lingjie]的文章
百度学术
百度学术中相似的文章
[Liu, Peng-fei]的文章
[Du, Yishuai]的文章
[Meng, Lingjie]的文章
必应学术
必应学术中相似的文章
[Liu, Peng-fei]的文章
[Du, Yishuai]的文章
[Meng, Lingjie]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Metabolic profiling in kidneys of Atlantic salmon infected with Aeromonas salmonicida based on H-1 NMR.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。