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Colorimetric detection of H2O2 using flower-like Fe-2(MoO4)(3) microparticles as a peroxidase mimic
Wang, Bing1; Ju, Peng2,3; Zhang, Dun4; Han, Xiuxun2,3; Zheng, Li5; Yin, Xiaofei1; Sun, Chengjun1,6
2016-11-01
发表期刊MICROCHIMICA ACTA
卷号183期号:11页码:3025-3033
文章类型Article
摘要The authors describe the preparation of Fe-2(MoO4)(3) materials with micrometer dimensions, and show that they are highly efficient peroxidase mimics that can be used in a photometric assay for H2O2. Depending on the experimental conditions of synthesis, the Fe-2(MoO4)(3) micromaterials display different morphologies, surface properties, and peroxidase-like activities. Among them, Fe-2(MoO4)(3) with flower-like structure has the largest BET specific surface area and the most negative potential. It exhibits the best peroxidase mimetic activity in catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to generate a blue-green product. Based on these findings, method was worked out for H2O2 determination that allows both visual and photometric read-out. The assay has a detection limit as low as 0.7 mu M, is selective and practical. Given its sensitivity, the method has a good potential with respect to determination of H2O2 in clinical samples but also in oxidase-type of reactions where H2O2 is being produced.
关键词Flower-like Structure Microparticles Xrd Fesem Edx Zeta Potential Kinetics Tmb Enzyme Mimic Bet Analysis
DOI10.1007/s00604-016-1955-8
收录类别SCI
语种英语
WOS记录号WOS:000386069400023
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文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136179
专题海洋腐蚀与防护研究发展中心
作者单位1.State Ocean Adm, Inst Oceanog 1, Marine Ecol Ctr, 6 Xianxialing Rd, Qingdao 266061, Peoples R China
2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lab Clean Energy Chem & Mat, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China
3.Qingdao Ctr Resource Chem & New Mat, 36 Jinshui Rd, Qingdao 266100, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Peoples R China
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GB/T 7714
Wang, Bing,Ju, Peng,Zhang, Dun,et al. Colorimetric detection of H2O2 using flower-like Fe-2(MoO4)(3) microparticles as a peroxidase mimic[J]. MICROCHIMICA ACTA,2016,183(11):3025-3033.
APA Wang, Bing.,Ju, Peng.,Zhang, Dun.,Han, Xiuxun.,Zheng, Li.,...&Sun, Chengjun.(2016).Colorimetric detection of H2O2 using flower-like Fe-2(MoO4)(3) microparticles as a peroxidase mimic.MICROCHIMICA ACTA,183(11),3025-3033.
MLA Wang, Bing,et al."Colorimetric detection of H2O2 using flower-like Fe-2(MoO4)(3) microparticles as a peroxidase mimic".MICROCHIMICA ACTA 183.11(2016):3025-3033.
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