| Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii | |
Li, Rongfeng1; Yu, Huahua1 ; Yue, Yang1,2; Liu, Song1; Xing, Ronge1; Chen, Xiaolin1; Li, Pengcheng1
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| 2016-10-04 | |
| 发表期刊 | JOURNAL OF PROTEOMICS
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| 卷号 | 148页码:57-64 |
| 文章类型 | Article |
| 摘要 | Jellyfish sting has become a worldwide issue of critical concern to human health and safety in coastal areas in recent decades. Cyanea nozakii is one of the dominant blooming species and dangerous stingers in China. However, it remains unclear how many and what types of toxins are present in the venom. So, we used a combined transcriptomics and proteomics approach to investigate the venom composition of jellyfish C. nozakii. In total 4,608,524 Illumina valid reads were obtained to de novo assemble to 40,434 unigenes in the transcriptomics analysis. And, a total of 311,635 MS/MS spectra with 12,247 unique MS/MS spectra were generated to 1556 homologous proteins in the proteomics analysis. 174 potential toxin proteins were identified, with 27 proteins homology to the toxins from venomous animals, including phospholipase A2, zinc metalloproteinase-disintegrin agkistin, serine protease inhibitor, plancitoxin-1, alpha-latrocrustotoxin-Ltla, etc. This study described the transcriptomics and venom proteomics of jellyfish C nozakii for the first time. Our findings provide a comprehensive understanding of the venom composition of C nozakii. Furthermore, the results may also be very helpful for the discovery of novel bioactive proteins, as well as the development of effective treatments for jellyfish sting in the future. |
| 关键词 | Jellyfish Cyanea Nozakii Toxin Venom Transcriptomics Proteomics |
| DOI | 10.1016/j.jprot.2016.07.023 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000385599700007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/136171 |
| 专题 | 海洋生物技术研发中心 实验海洋生物学重点实验室 海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuguan Rd, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Rongfeng,Yu, Huahua,Yue, Yang,et al. Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii[J]. JOURNAL OF PROTEOMICS,2016,148:57-64. |
| APA | Li, Rongfeng.,Yu, Huahua.,Yue, Yang.,Liu, Song.,Xing, Ronge.,...&Li, Pengcheng.(2016).Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii.JOURNAL OF PROTEOMICS,148,57-64. |
| MLA | Li, Rongfeng,et al."Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii".JOURNAL OF PROTEOMICS 148(2016):57-64. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Combined proteomics （1869KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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