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Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii
Li, Rongfeng1; Yu, Huahua1; Yue, Yang1,2; Liu, Song1; Xing, Ronge1; Chen, Xiaolin1; Li, Pengcheng1
2016-10-04
Source PublicationJOURNAL OF PROTEOMICS
Volume148Pages:57-64
SubtypeArticle
AbstractJellyfish sting has become a worldwide issue of critical concern to human health and safety in coastal areas in recent decades. Cyanea nozakii is one of the dominant blooming species and dangerous stingers in China. However, it remains unclear how many and what types of toxins are present in the venom. So, we used a combined transcriptomics and proteomics approach to investigate the venom composition of jellyfish C. nozakii. In total 4,608,524 Illumina valid reads were obtained to de novo assemble to 40,434 unigenes in the transcriptomics analysis. And, a total of 311,635 MS/MS spectra with 12,247 unique MS/MS spectra were generated to 1556 homologous proteins in the proteomics analysis. 174 potential toxin proteins were identified, with 27 proteins homology to the toxins from venomous animals, including phospholipase A2, zinc metalloproteinase-disintegrin agkistin, serine protease inhibitor, plancitoxin-1, alpha-latrocrustotoxin-Ltla, etc. This study described the transcriptomics and venom proteomics of jellyfish C nozakii for the first time. Our findings provide a comprehensive understanding of the venom composition of C nozakii. Furthermore, the results may also be very helpful for the discovery of novel bioactive proteins, as well as the development of effective treatments for jellyfish sting in the future.
KeywordJellyfish Cyanea Nozakii Toxin Venom Transcriptomics Proteomics
DOI10.1016/j.jprot.2016.07.023
Indexed BySCI
Language英语
WOS IDWOS:000385599700007
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136171
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
海洋环境工程技术研究发展中心
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, 19 Yuguan Rd, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Rongfeng,Yu, Huahua,Yue, Yang,et al. Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii[J]. JOURNAL OF PROTEOMICS,2016,148:57-64.
APA Li, Rongfeng.,Yu, Huahua.,Yue, Yang.,Liu, Song.,Xing, Ronge.,...&Li, Pengcheng.(2016).Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii.JOURNAL OF PROTEOMICS,148,57-64.
MLA Li, Rongfeng,et al."Combined proteomics and transcriptomics identifies sting-related toxins of jellyfish Cyanea nozakii".JOURNAL OF PROTEOMICS 148(2016):57-64.
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