Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino

doi:10.1016/j.aquaculture.2016.08.010

IOCAS-IR > 海洋生物技术研发中心

	Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino
	Gao Xiaolong1,2 ; Li Xian 2; Li Meijie3 ; Song Changbin 4; Liu Ying2
	2016-12-01
发表期刊	AQUACULTURE
卷号	465 页码:78-87
文章类型	Article
摘要	We examined the effects of light intensity on the metabolism, antioxidant enzymes, and expression of related genes in Haliotis discus hannai Ino, to determine the optimal lighting conditions for aquaculture of these animals. Succinate dehydrogenase activity was significantly lower after 48 h in animals exposed to light intensities of 30 and 60 mu mol/m(2)/s, while lactate dehydrogenase activity and lactic acid content at 96 h were significantly higher compared with animals in the dark or exposed to 5 or 15 mu mol/m(2)/s (both P < 0.05). There was no significant difference in reactive oxygen species (ROS) contents between animals in the dark and those exposed to 5 or 15 mu mol/m(2)/s (P > 0.05) throughout the experiment. However, ROS contents under light intensities of 30 and 60 mu mol/m(2)/s peaked at 12 h, when expression levels of genes encoding catalase (CAT), thioredoxin peroxidase (TPX), sigma-glutathione-s-transferase (GST(S)), and mu-glutathione-s-transferase (GST(m)) also began to increase, and CAT and glutathione peroxidase (GP(X)) activities were significantly higher than in animals in the dark or exposed to 5 or 15 mu mol/m(2)/s (P < 0.05). After a further 12 h, the ROS content at 30 mu mol/m(2)/s began to decrease, and GPX activity and malondialdehyde (MDA) content also gradually decreased, followed by increases in ROS and MDA 48 h later, to reach respective peaks at 96 h. Gene expression levels of TPX, GST(S), GST(m), heat shock protein 70 (HSP70), and CAT began to decrease 72 h later, suggesting that excessive accumulation of ROS may have caused oxidative damage to the tissues. At a light intensity of 60 mu mol/m(2)/s, expression levels of TPX, GST(S), GST(m), and HSP70 genes, total antioxidant capacity, and GP(X) activity peaked at 48 h, and the ROS content was significantly higher than in any other group 48 h later (P < 0.05). However, gene expression levels of TPX, GST(S), GST(m), HSP70, HSP90, and CAT, and reduced glutathione content began to decrease, indicating that the antioxidant system had been unable to perform its normal physiological functions to withstand the long-term stress of adverse environmental factors. These results suggest that the light intensity should be controlled at 5-15 mu mol/m(2)/s during H. discus hannai aquaculture, to maintain the organism's normal physiological metabolism.
关键词	Haliotis Discus Hannai Ino Light Intensity Antioxidant Enzyme Heat Shock Protein Real-time Quantitative Pcr
DOI	10.1016/j.aquaculture.2016.08.010
收录类别	SCI
语种	英语
WOS记录号	WOS:000385372200011
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/136166
专题	海洋生物技术研发中心实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc, Qingdao 266101, Peoples R China 4.Chinese Acad Sci, Inst Semicond, Beijing 100083, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gao Xiaolong,Li Xian,Li Meijie,et al. Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino[J]. AQUACULTURE,2016,465:78-87.
APA	Gao Xiaolong,Li Xian,Li Meijie,Song Changbin,&Liu Ying.(2016).Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino.AQUACULTURE,465,78-87.
MLA	Gao Xiaolong,et al."Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino".AQUACULTURE 465(2016):78-87.

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