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Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in vivo and in vitro
Sha, Yujie1,2,3; Wang, Baojie1,2; Liu, Mei1,2; Jiang, Keyong1,2; Wang, Lei1,2
2016-12-01
Source PublicationAQUACULTURE
Volume465Pages:117-123
SubtypeArticle
AbstractIn this study, we investigated the interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 when cocultured in Litopenaeus vannamei in vivo and in vitro. Fluorescence microscopy was used to observe the distribution of these two strains, which were labeled with DAPI and CFDA-SE, respectively, in the gut of the shrimp. Fluorescent imaging revealed that L. pentosus HC-2 competitively excluded V. parahaemolyticus E1 in the intestine of L. vannamei when the shrimp diet contained both these strains. The growth of both bacteria and the expression of quorum-sensing-and virulence-related genes of L. pentosus HC-2 or V. parahaemolyticus E1 were monitored when they were cultured alone or together in vitro. In contrast to L. pentosus HC-2, the population density of V. parahaemolyticus E1 decreased significantly after coculture for 8 h. A transcriptional analysis showed that the expression of the luxS gene in L. pentosus HC-2 increased after 8 h when it was cocultured with V. parahaemolyticus E1, suggesting that LuxS is involved in the competitive exclusion of V. parahaemolyticus E1 by L. pentosus HC-2. Interestingly, the aphA, opaR, and tlh genes of V. parahaemolyticus E1 were significantly upregulated in response to heat-killed L. pentosus HC-2 cells. This suggests that the intracellular components of L. pentosus HC-2 induce the expression of toxicity-related factors in V. parahaemolyticus E1 for a short time in coculture.
KeywordLactobacillus Pentosus Hc-2 Vibrio Parahaemolyticus E1 Quorum Sensing Competitive Exclusion Gene Expression
DOI10.1016/j.aquaculture.2016.09.007
Indexed BySCI
Language英语
WOS IDWOS:000385372200017
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136158
Collection海洋生物技术研发中心
实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sha, Yujie,Wang, Baojie,Liu, Mei,et al. Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in vivo and in vitro[J]. AQUACULTURE,2016,465:117-123.
APA Sha, Yujie,Wang, Baojie,Liu, Mei,Jiang, Keyong,&Wang, Lei.(2016).Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in vivo and in vitro.AQUACULTURE,465,117-123.
MLA Sha, Yujie,et al."Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in vivo and in vitro".AQUACULTURE 465(2016):117-123.
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