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Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in vivo and in vitro
Sha, Yujie1,2,3; Wang, Baojie1,2; Liu, Mei1,2; Jiang, Keyong1,2; Wang, Lei1,2
2016-12-01
发表期刊AQUACULTURE
卷号465页码:117-123
文章类型Article
摘要In this study, we investigated the interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 when cocultured in Litopenaeus vannamei in vivo and in vitro. Fluorescence microscopy was used to observe the distribution of these two strains, which were labeled with DAPI and CFDA-SE, respectively, in the gut of the shrimp. Fluorescent imaging revealed that L. pentosus HC-2 competitively excluded V. parahaemolyticus E1 in the intestine of L. vannamei when the shrimp diet contained both these strains. The growth of both bacteria and the expression of quorum-sensing-and virulence-related genes of L. pentosus HC-2 or V. parahaemolyticus E1 were monitored when they were cultured alone or together in vitro. In contrast to L. pentosus HC-2, the population density of V. parahaemolyticus E1 decreased significantly after coculture for 8 h. A transcriptional analysis showed that the expression of the luxS gene in L. pentosus HC-2 increased after 8 h when it was cocultured with V. parahaemolyticus E1, suggesting that LuxS is involved in the competitive exclusion of V. parahaemolyticus E1 by L. pentosus HC-2. Interestingly, the aphA, opaR, and tlh genes of V. parahaemolyticus E1 were significantly upregulated in response to heat-killed L. pentosus HC-2 cells. This suggests that the intracellular components of L. pentosus HC-2 induce the expression of toxicity-related factors in V. parahaemolyticus E1 for a short time in coculture.
关键词Lactobacillus Pentosus Hc-2 Vibrio Parahaemolyticus E1 Quorum Sensing Competitive Exclusion Gene Expression
DOI10.1016/j.aquaculture.2016.09.007
收录类别SCI
语种英语
WOS记录号WOS:000385372200017
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/136158
专题海洋生物技术研发中心
实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Sha, Yujie,Wang, Baojie,Liu, Mei,et al. Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in vivo and in vitro[J]. AQUACULTURE,2016,465:117-123.
APA Sha, Yujie,Wang, Baojie,Liu, Mei,Jiang, Keyong,&Wang, Lei.(2016).Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in vivo and in vitro.AQUACULTURE,465,117-123.
MLA Sha, Yujie,et al."Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in vivo and in vitro".AQUACULTURE 465(2016):117-123.
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