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An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway
Chen, Hao1,3; Wang, Hao1; Jiang, Shuai1; Xu, Jiachao1,3; Wang, Lingling2; Qiu, Limei1; Song, Linsheng2
2016-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume57Pages:160-169
SubtypeArticle
AbstractmiRNAs are important gene regulators at post-transcriptional level and can modulate diverse biological processes, including immune response. Dozens of species-specific miRNAs have been identified in oyster Crassostrea gigas while their functions remain largely unknown. In the present study, an oyster species specific miRNA scaffold42648_5080 was found responsive to LPS stimulation and might target a total of 31 oyster genes possibly involved in cell communication, cellular localization and cellular response to stimulus. Besides, in gain-of-function assay of scaffold42648_5080 in vivo, the phagocytosis (30.90% in miRNA group verse 23.20% in miRNA control group), apoptosis (3.10% in miRNA group verse 5.30% in miRNA control group) and migration rate (13.88% in miRNA group verse 21.03% in miRNA control group) of oyster haemocytes were found significantly altered after the injection of scaffold42648_5080 mimics. Among the target genes, integrin-linked kinase (CgILK) was considered crucial in cell migration and its interaction with scaffold42648_5080 was then verified both in vitro and in vivo. Consequently, a significant decrease of relative luciferase ratio was observed in CgILK 3'-UTR luciferase reporter assay after transfection of scaffold42648_5080 mimics (0.70-fold of that in blank group, p < 0.01). Meanwhile, when scaffold42648_5080 was overexpressed in vivo (5.41-fold of miRNA control group, p < 0.01), the expression of CgILK declined significantly to 0.25-fold of miRNA control group (p < 0.01). Comparatively, a significant decrease of the haemocyte migration rate (19.76% verse 34.82% in siEGFP control group, p < 0.01) was observed after knock-down of CgILK in vivo. The present study, as far as we know, for the first time revealed the immunomodulation role of an oyster species-specific miRNA, which might provide new insights into miRNA-mediated adaptation mechanism of oysters. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordSpecies-specific Mirna Cellular Immune Response Integrin-linked Kinase Integrin Pathway Cell Migration
DOI10.1016/j.fsi.2016.08.032
Indexed BySCI
Language英语
WOS IDWOS:000385327100017
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/136154
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Hao,Wang, Hao,Jiang, Shuai,et al. An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway[J]. FISH & SHELLFISH IMMUNOLOGY,2016,57:160-169.
APA Chen, Hao.,Wang, Hao.,Jiang, Shuai.,Xu, Jiachao.,Wang, Lingling.,...&Song, Linsheng.(2016).An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway.FISH & SHELLFISH IMMUNOLOGY,57,160-169.
MLA Chen, Hao,et al."An oyster species-specific miRNA scaffold42648_5080 modulates haemocyte migration by targeting integrin pathway".FISH & SHELLFISH IMMUNOLOGY 57(2016):160-169.
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